详情页 - 聚合机构库演示平台

当前位置：首页 > 详情页

A method for constructing a mouse model of congenital hearing loss by bilateral cochlear ablation.

81| 认领 | 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

单位： [1]Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Clinical Center for Hearing Loss, Capital Medical University, Beijing 100050, China. Electronic address: liyang1218@mail.ccmu.edu.cn. [2]Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Clinical Center for Hearing Loss, Capital Medical University, Beijing 100050, China. Electronic address: 19931331074@163.com. [3]Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Clinical Center for Hearing Loss, Capital Medical University, Beijing 100050, China. Electronic address: tengqi@mail.ccmu.edu.cn. [4]Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Clinical Center for Hearing Loss, Capital Medical University, Beijing 100050, China. Electronic address: gongss@ccmu.edu.cn. [5]Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Clinical Center for Hearing Loss, Capital Medical University, Beijing 100050, China. Electronic address: liuke@ccmu.edu.cn.

出处：

DOI：

ISSN：

摘要：

Cochlear ablation is one of the most classic methods for the animal model establishment of hearing loss (HL), but the vast majority of rodents taken by cochlear ablation have been rats and adult mice so far. Given the importance of mice in HL studies, it is urgently necessary to expand the application of cochlear ablation in mice at different ages.Bilateral cochlear ablation surgery was performed in C57BL/6 J mice before the onset of hearing at postnatal (P) day 10. Auditory brainstem response was performed to evaluate the hearing of mice. Open field test and swimming test were performed to examine the vestibular function of mice.A permanent HL model was built by bilateral cochlear ablation in C57BL/6 J mice at P10. This method could successfully destroy cochlear hair cells and cause no vestibular deterioration in the adulthood of mice.In addition to transgenic mice, permanently deaf mice used in this field in the existing studies are restricted to a time point after their ear canal opening. This study is the first to establish a congenital and permanent HL mouse model by cochlear ablation without gene modification.Bilateral cochlear ablation in mice at P10 is an ideal and effective way for congenital HL mice model foundation, and the vestibular function seems not to be deteriorated.Copyright © 2022 Elsevier B.V. All rights reserved.

语种：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2021]版：

大类 | 4 区医学

小类 | 4 区生化研究方法 4 区神经科学

最新[2025]版：

大类 | 4 区医学

小类 | 3 区生化研究方法 4 区神经科学

JCR分区：

出版当年[2020]版：

Q3 BIOCHEMICAL RESEARCH METHODS Q4 NEUROSCIENCES

最新[2023]版：

Q2 BIOCHEMICAL RESEARCH METHODS Q3 NEUROSCIENCES

影响因子： 最新[2023版] 2.7 最新五年平均[2021-2025] 2.7 出版当年[2020版] 2.39 出版当年五年平均[2016-2020] 3.183 出版前一年[2019版] 2.214 出版后一年[2021版] 2.987

第一作者：

第一作者单位： [1]Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Clinical Center for Hearing Loss, Capital Medical University, Beijing 100050, China. Electronic address: liyang1218@mail.ccmu.edu.cn.

推荐引用方式(GB/T 7714)：

APA：

MLA：