单位：[1]Beijing Univ Chinese Med, Dongfang Hosp, Dept Nephrol, Beijing 100078, Peoples R China[2]China Japan Friendship Hosp, Inst Clin Med, Beijing Key Lab Immunoinflammatory Dis, Beijing 100029, Peoples R China[3]Beijing Univ Chinese Med, Dongfang Hosp, Dept Internal Med Western Med, Beijing 100078, Peoples R China[4]Beijing Univ Chinese Med, Hemodialysis Dept, Dongfang Hosp, Beijing 100078, Peoples R China[5]Beijing Hosp Tradit Chinese Med, Shunyi Hosp, Off Acad Res, Beijing 101300, Peoples R China[6]Beijing Univ Chinese Med, Beijing 100029, Peoples R China
Objective: To explore the effect of Tangshen formula on the oxidative renal injury in UUO mice. Method: Seven-week-old male mice C57BL/6N were selected and randomly divided into sham-surgery group, UUO model group (UUO-7d and UUO-14d), Tangshen formula (TF) group (UUO + TF-7d and UUO + TF-14d) and fosinopril group (UUO + fosinopril-7d, UUO + fosinopril-14d). The model were established after 5d medication, and continuous postoperative medications for 7d or 14d were also carried out. The renal pathology was observed, the MDA concentration and SOD activity of renal tissue and serum were evaluated, along with the expression of NADPH oxidase in renal tissue. Result: Compared with that of sham-surgery group, the inflammatory cells in renal interstitium of UUO mice is characterized by diffuse infiltration and dramatical proliferation of collagen fibers, while the introduction of Tangshen formula helps to alleviate this situation, which decreases MDA concentration (all P < 0.001 for renal tissue and serum) but increases SOD activity (P < 0.001 in renal tissue, P < 0.05 in serum), as well as downregulates the expression of Nox4 (P < 0.001) and phosphorylated p47phox (P < 0.001) in renal tissue. Conclusion: Tangshen formula alleviates the pathological injury of renal tissue in UUO mice and fibrosis of renal interstitium, the underlying mechanism is relative to the downregulated expression of NADPH oxidase, upon which the oxidative stress level of kidney and serum in UUO mice is decreased.
基金：
Beijing University of traditional Chinese Med-icine [2019-JYB-JS-090]
第一作者单位：[1]Beijing Univ Chinese Med, Dongfang Hosp, Dept Nephrol, Beijing 100078, Peoples R China
通讯作者：
通讯机构：[1]Beijing Univ Chinese Med, Dongfang Hosp, Dept Nephrol, Beijing 100078, Peoples R China[*1]Beijing Univ Chinese Med, Dongfang Hosp, 6 Fangxingyuan First Dist, Beijing, Peoples R China
推荐引用方式(GB/T 7714)：
Ding Xinyu,Li Ping,Tan Chao,et al.Effect of Tangshen formula on alleviating oxidative renal injury in UUO mice by downregulated expression of NADPH oxidase[J].JOURNAL of RADIATION RESEARCH and APPLIED SCIENCES.2022,15(3):65-71.doi:10.1016/j.jrras.2022.05.007.
APA：
Ding, Xinyu,Li, Ping,Tan, Chao,Wang, Liwei,Liu, Peng...&Zhang, Chengcheng.(2022).Effect of Tangshen formula on alleviating oxidative renal injury in UUO mice by downregulated expression of NADPH oxidase.JOURNAL of RADIATION RESEARCH and APPLIED SCIENCES,15,(3)
MLA：
Ding, Xinyu,et al."Effect of Tangshen formula on alleviating oxidative renal injury in UUO mice by downregulated expression of NADPH oxidase".JOURNAL of RADIATION RESEARCH and APPLIED SCIENCES 15..3(2022):65-71
