详情页 - 聚合机构库演示平台

当前位置：首页 > 详情页

Development and Clinical Application of a Rapid and Visual Loop-Mediated Isothermal Amplification Test for tetM gene in Clostridioides difficile Strains Cultured from Feces

56| 认领 | 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

单位： [1]Department of Infectious Diseases, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, 5190 0 0, China [2]Department of Gastroenterology, Guangdong Provincial Key Laboratory of Gastroenterology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou, China [3]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, Beijing, 10 0 050, China [4]Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 10 0 071, China [5]Department of Gastroenterology, Integrative Microecology Center, Shenzhen Hospital, Southern Medical University, Shenzhen, 518100, China

出处：

DOI：

ISSN：

摘要：

To develop a rapid and visual loop-mediated isothermal amplification (LAMP) assay targeting the tetM gene in Clostridioides difficile (C. difficile) strains cultured from feces.Primers were designed to recognize the tetM gene in C. difficile by LAMP, using turbidity and visual detection. The sensitivity and specificity of LAMP primers was determined. Besides, We conducted both LAMP and polymerase chain reaction (PCR) for the tcdA, tcdB, cdtA, cdtB, ermB, tetM genes in 300 toxigenic C. difficile strains cultured from feces.The target DNA was amplified and visualized within 60 minutes at a temperature of 62°C. A total of 26 bacterial strains were found negative for tetM, which manifested high specificity of the primers. The detection limit of LAMP was 36.1 pg/µl, which was 100-fold more sensitive than PCR. The positive rate of tetM in toxigenic C. difficile strains cultured from feces was 93.3% by both LAMP and PCR. The proportion of toxin types in those C. difficile strains was 95.7% for A+B+CDT-, 4% for A-B+CDT-, and 0.3% for A+B+CDT+, respectively.This is the first study examining tetM gene in C. difficile strains cultured from feces by LAMP. Its high specificity and sensitivity, as well as visual detection, make the new assay a powerful diagnostic tool for rapid testing.Copyright © 2022. Published by Elsevier Ltd.

基金：

语种：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2021]版：

大类 | 3 区医学

小类 | 3 区传染病学

最新[2025]版：

大类 | 2 区医学

小类 | 2 区传染病学

JCR分区：

出版当年[2020]版：

Q2 INFECTIOUS DISEASES

最新[2023]版：

Q1 INFECTIOUS DISEASES

影响因子： 最新[2023版] 4.8 最新五年平均[2021-2025] 5 出版当年[2020版] 3.623 出版当年五年平均[2016-2020] 4.01 出版前一年[2019版] 3.202 出版后一年[2021版] 12.073

第一作者：

第一作者单位： [1]Department of Infectious Diseases, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, 5190 0 0, China

共同第一作者：

通讯作者：

通讯机构： [1]Department of Infectious Diseases, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, 5190 0 0, China [5]Department of Gastroenterology, Integrative Microecology Center, Shenzhen Hospital, Southern Medical University, Shenzhen, 518100, China [*1]Department of Gastroenterology, Integrative Microecology Center, Shenzhen Hospital, Southern Medical University, Shenzhen, 518100, China. [*2]Department of Infectious Diseases, The Fifth Affiliated Hospital, Sun Yat-Sen University, 52 East Meihua Road, Zhuhai, 5190 0 0, China.

推荐引用方式(GB/T 7714)：

APA：

MLA：