单位：[1]Department of Ultrasound, Aero Space Central Hospital, Beijing 100050, China.[2]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing 100050, China.临床科室国家中心消化分中心消化内科首都医科大学附属北京友谊医院
Autophagy-related genes (ATGs) play critical roles in tumorigenesis and progression in gastric cancer (GC). The present study aimed to identify immune-based prognostic ATGs and verify their functions in tumor immune microenvironment (TIME) in GC. Macrophage infiltration was found to negatively correlate with prognosis in GC patients. After stratifying by infiltration levels of macrophages, we screened The Cancer Genome Atlas and Human Autophagy Database to identify the differentially expressed ATGs (DE-ATGs). Of 1,433 differentially expressed genes between the two groups, seven genes qualified as DE-ATGs. Of these, CXCR4, DLC1, and MAP1LC3C, exhibited strong prognostic prediction ability in Kaplan-Meier survival-log-rank test. High expression of these genes correlated with increased occurrence of advanced grade 3 tumors and poor prognoses. Furthermore, GSEA indicated that they were significantly associated with oncogenic and immune-related pathways. The comprehensive evaluation of TIME via GEPIA, ESTIMATE, CIBERSORT, and TIMER suggested that the three DE-ATGs were closely associated with immune condition, both in terms of immune cells and immune scores. Thus, the outcome of this study may aid in better understanding of the ATGs and their interaction with the immune microenvironment, which would allow the development of novel inhibitors, personalized treatment, and immunotherapy in gastric cancer.
基金：
Hospital level project
of Aerospace Center Hospital (YN202105), Beijing
Association of Science and Technology Golden Bridge
Project Seed Fund (ZZ21054), Aerospace Medical and
Health Science and Technology Group, (2021YK01)
and Scientific research and cultivation plan of health
development in Haidian District (HP2021-32-50702).
语种：
外文
WOS：
PubmedID：
第一作者：
第一作者单位：[1]Department of Ultrasound, Aero Space Central Hospital, Beijing 100050, China.[2]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing 100050, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Tian Ruyue,Sun Ya,Han Xuedi,et al.Identification and validation of prognostic autophagy-related genes associated with immune microenvironment in human gastric cancer[J].Aging.2022,14(18):7617-7634.doi:10.18632/aging.204313.
APA：
Tian Ruyue,Sun Ya,Han Xuedi,Wang Jiajun,Gu Hongli...&Liang Lei.(2022).Identification and validation of prognostic autophagy-related genes associated with immune microenvironment in human gastric cancer.Aging,14,(18)
MLA：
Tian Ruyue,et al."Identification and validation of prognostic autophagy-related genes associated with immune microenvironment in human gastric cancer".Aging 14..18(2022):7617-7634
