单位：[1]Stomatology Department,Peking University Third Hospital, Beijing 100191, China.[2]Department of Stomatology,Peking University Third Hospital Yanqing Hospital, Beijing, China.[3]Department of Periodontology, School/Hospital of Stomatology, Lanzhou University, Lanzhou, Gansu Province, China.[4]Department of Pediatric Dentistry, School and Hospital of Stomatology, Sun Yat-Sen University, Guangzhou,Guangdong Province, China.[5]Dermatology Department,Peking University First Hospital,Beijing, China.[6]Oral Medical Center, Shenzhen People’s Hospital,Shen Zhen, Guangdong Province, China.深圳市人民医院深圳医学信息中心[7]Department of Oral Medicine, Peking University School and Hospital of Stomatology,Beijing,China.[8]Department of Stomatology, China-Japan Friendship Hospital, Beijing,China.[9]Stomatological Experimental Center, Dental Hospital Afliated to Jiamusi University, Jiamusi, Heilongjiang Province, China.[10]National Institute on Drug Dependence, Peking University and Beijing Key Laboratory of Drug Dependence Research, Peking University, Beijing 100191, China.
Lichen planus (LP) is a chronic inflammatory disease. Oral lichen planus (OLP) mainly appears as oral mucosal reticular or ulcerative lesions with an unknown etiology. We aimed to explore the immunomodulatory effect of paeoniflorin (PF) in mesenchymal stem cells (MSCs) and the potential involvement of Th1/Th2 cytokines in OLP. The effects of paeoniflorin on the proliferation and migration of MSCs were detected by Cell Counting Kit-8 (CCK8) and Transwell assays. MSCs were subjected to osteogenic, adipogenic and neurogenic induction followed by Alizarin red, oil red O, real-time PCR and immunofluorescence assays. We found that paeoniflorin promoted the proliferation, migration and multilineage differentiation of MSCs from OLP lesions (OLP-MSCs) in vitro. Paeoniflorin pretreatment increased the inhibitory effect of OLP-MSCs on peripheral blood mononuclear cells. Furthermore, paeoniflorin-pretreated OLP-MSCs simultaneously decreased Th1 cytokine levels and increased Th2 cytokine levels in T lymphocyte cocultures. Finally, paeoniflorin-pretreated OLP-MSCs also promoted the Th1/Th2 balance both in vitro and in the serum of mice that received skin allografts. In conclusion, paeoniflorin enhanced MSC immunomodulation and changed the inflammatory microenvironment via T lymphocytes, suggesting that the improvement of OLP-MSCs is a promising therapeutic approach for OLP.
基金：
Tis work was supported by grants from the National Natural Science Foundation of China (No. 81800983), the
Peking University Medicine Seed Fund for Interdisciplinary Research (No. BMU2020MX022, 71006Y2337), the
Key Clinical Projects of Peking University Tird Hospital (No. BYSYZD2019035), and the State Key Laboratory
of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese
Academy of Sciences (KF2020-18).
