单位：[1]Gastrointestinal Surgery Department, China-Japan Friendship Hospital, Beijing, China[2]Nanchang Institute of Technology, College of Medicine, China. Pooling Medical Research Institutes, Hangzhou, China[3]Pooling Medical Research Institutes, Hangzhou, Beijing, China[4]Department of Pathology, East Hospital, Tongji University, Shanghai, China[5]Gastrointestinal Surgery, Peking University International Hospital, Beijing, China[6]Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing, China
Gastric cancer (GC) is a cancer with a high mortality rate. lncRNAs play a role in regulating GC tumorigenesis. In this paper, we analyzed differentially expressed lncRNAs between GC and adjacent normal tissues using multiple bioinformatics tools to identify new potential targets in GC. Cell viability and migration ability were detected using the Cell Counting Kit-8 (CCK-8) and transwell assays, MIR4435-2HG was negatively correlated with the survival rate of GC patients, and by inhibiting the activity of MIR4435-2HG, the viability and migration ability of GC cells could be reduced. In addition, RT- qPCR and western blot to detect gene and protein level expression, transmission electron microscopy and nanoparticle tracking analysis (NTA) to study the efficiency of exosome isolation, and flow cytometry to observe cell differentiation were employed, delivery of MIR4435-2HG shRNA via MKN45 cell-derived exosomes significantly reversed the MKN45 exosome-induced M2 polarization in macrophages. Furthermore, the low expression of MIR4435-2HG in MKN45 cell-derived exosomes inhibited the Jagged1/Notch and JAK1/STAT3 pathways in macrophages; MIR4435-2HG downregulated exosomes were found to significantly inhibit GC tumor growth in vivo by establishing a mouse model. In short, MKN45 cell-derived exosomes deliver lncRNA MIR4435-2HG, which promotes gastric carcinogenesis by inducing macrophage M2 polarization.
基金：
National Natural Science Foundation of China; [82002477]
第一作者单位：[1]Gastrointestinal Surgery Department, China-Japan Friendship Hospital, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]Gastrointestinal Surgery Department, China-Japan Friendship Hospital, Beijing, China[6]Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing, China
推荐引用方式(GB/T 7714)：
Li Chaofeng,Chen Zhengju,Gao Jinli,et al.MIR4435-2HG in exosomes promotes gastric carcinogenesis by inducing M2 polarization in macrophages[J].FRONTIERS IN ONCOLOGY.2022,12:doi:10.3389/fonc.2022.1017745.
APA：
Li, Chaofeng,Chen, Zhengju,Gao, Jinli,Tang, Tao,Zhou, Lei...&Fu, Tao.(2022).MIR4435-2HG in exosomes promotes gastric carcinogenesis by inducing M2 polarization in macrophages.FRONTIERS IN ONCOLOGY,12,
MLA：
Li, Chaofeng,et al."MIR4435-2HG in exosomes promotes gastric carcinogenesis by inducing M2 polarization in macrophages".FRONTIERS IN ONCOLOGY 12.(2022)
