Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury
单位：[1]Department of Urology, Beijing Friendship Hospital, Beijing, China临床科室泌尿外科泌尿外科首都医科大学附属北京友谊医院[2]School of Rehabilitation Medicine, Capital Medical University, Beijing, China, [3]China Rehabilitation Science Institute, Beijing, China, [4]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China, [5]Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China, [6]Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China, [7]Department of Rehabilitation Medicine, The Second Hospital of Anhui Medical University, Hefei, China
IntroductionSpinal cord injury (SCI) often causes continuous neurological damage to clinical patients. Circular RNAs (circRNAs) are related to a lot of diseases, including SCI. We previously found five candidate circRNAs which were likely to regulate the secondary pathophysiological changes in rat model after traumatic SCI. MethodsIn this study, we first selected and overexpressed target circRNA in rats. We then explored its functional roles using various functional assays in a rat model after SCI. ResultsWe found that rno-circRNA-013017-the selected target circRNA-reduced neuron apoptosis, preserved the survival and activity of motor neurons, and regulated apoptosis-related proteins at 3 days post-SCI using western blot, immunofluorescence and polymerase chain reaction. Additionally, we found that rno-circRNA-013017 inhibited descending axonal degeneration and preserved motor neurons and descending axons at 6 weeks post-SCI using immunofluorescence, biotin dextran amine diffusion tensor imaging. Finally, the overexpression of rno-circRNA-013017 promoted the locomotor function of rats after SCI using open-field test and gait analysis. ConclusionFocusing on the functions of rno-circRNA-013017, this study provides new options for future studies exploring therapeutic targets and molecular mechanisms for SCI.
基金：
This study was supported by grants from the
National Natural Science Foundation of China (General
Program 81870979), the National Key R&D Program
of China (2018YFF0301104), and the Basic Scientific
Research Foundation of China Rehabilitation Research
Center (2018ZX-30).
第一作者单位：[1]Department of Urology, Beijing Friendship Hospital, Beijing, China[2]School of Rehabilitation Medicine, Capital Medical University, Beijing, China, [3]China Rehabilitation Science Institute, Beijing, China, [4]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China, [5]Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China, [6]Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China,
共同第一作者：
通讯作者：
通讯机构：[2]School of Rehabilitation Medicine, Capital Medical University, Beijing, China, [3]China Rehabilitation Science Institute, Beijing, China, [4]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China, [5]Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China, [6]Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China,
推荐引用方式(GB/T 7714)：
Qin Chuan,Liu Yi,Xu Pei-Pei,et al.Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury[J].FRONTIERS IN NEUROSCIENCE.2022,16:doi:10.3389/fnins.2022.1065897.
APA：
Qin, Chuan,Liu, Yi,Xu, Pei-Pei,Zhang, Xin,Talifu, Zuliyaer...&Li, Jian-Jun.(2022).Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury.FRONTIERS IN NEUROSCIENCE,16,
MLA：
Qin, Chuan,et al."Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury".FRONTIERS IN NEUROSCIENCE 16.(2022)
