Immune responses and protective effects against Japanese encephalitis induced by a DNA vaccine encoding the prM/E proteins of the attenuated SA14-14-2 strain
单位：[1]Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China 首都医科大学附属北京友谊医院[2]Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Beijing 100050, China [3]Department of Gastroenterology, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China 首都医科大学附属北京儿童医院[4]Outpatient Department, Beijing Friendship Hospital, Capital Medical University, Beijing 100050 China 首都医科大学附属北京友谊医院[5] State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China [6]Beijing Key Laboratory of Pediatric Respiratory Infection diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Research Unit of Critical Infection in Children, Chinese Academy of Medical Sciences, 2019RU016, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, National Center for Children’s Health, Capital Medical University, Beijing 100045, China 首都医科大学附属北京儿童医院[7]Department of Internal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China
Japanese encephalitis virus (JEV) is the causal pathogen of Japanese encephalitis (JE), which has become a severe public health problem and is one of the most rapidly spreading mosquito-borne diseases worldwide. Currently, there is no specific treatment for JEV. A vaccine would be an effective measure for reducing morbidity and mortality. Although the live attenuated vaccine SA14-14-2 has been approved in some countries, it is still necessary to develop safer, more effective, and less costly vaccines. In this study, a DNA vaccine candidate, pV-SA14ME, expressing the prM/E proteins of SA14-14-2 was inoculated into BALB/c mice via intramuscular electroporation, and the immunogenicity and degree of protection were evaluated. We found that administration of 50 mu g pV-SA14ME via electroporation via three immunizations could induce persistent humoral and cellular immune responses and effectively protect mice against lethal JEV challenge. This study provides a basis for the subsequent promotion and use of the vaccine and lays the foundation for its further use in swine and humans.
基金：
Cultivation Fund Project of the National Natural Science Foundation at Beijing Children's Hospital, Capital Medical University [GPQN201909]; Research Foundation of Capital Medical University [PYZ2018052]; Startup Fund of Beijing Friendship Hospital, Capital Medical University [Yyqdkt2016-24]
第一作者单位：[1]Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China [2]Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Beijing 100050, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zheng Xiaoyan,Yu Xiaozheng,Wang Yan,et al.Immune responses and protective effects against Japanese encephalitis induced by a DNA vaccine encoding the prM/E proteins of the attenuated SA14-14-2 strain[J].INFECTION GENETICS and EVOLUTION.2020,85:doi:10.1016/j.meegid.2020.104443.
APA：
Zheng Xiaoyan,Yu Xiaozheng,Wang Yan,Cui Min,Wang Ran&Yin Chenghong.(2020).Immune responses and protective effects against Japanese encephalitis induced by a DNA vaccine encoding the prM/E proteins of the attenuated SA14-14-2 strain.INFECTION GENETICS and EVOLUTION,85,
MLA：
Zheng Xiaoyan,et al."Immune responses and protective effects against Japanese encephalitis induced by a DNA vaccine encoding the prM/E proteins of the attenuated SA14-14-2 strain".INFECTION GENETICS and EVOLUTION 85.(2020)
