单位：[1]Department of Stomatology, Beijing Friendship Hospital,Capital Medical University, Beijing, China临床科室口腔科口腔科首都医科大学附属北京友谊医院[2]Department of Stomatology, Beijing Electric PowerHospital, Beijing, China[3]Xiangya Stomatology Hospital and School of Stomatology,Central South University, Changsha, Hunan, China[4]Capital Medical University School of Stomatology, No. 4Tian Tan Xi Li, Beijing, China
Objective To investigate the function of enhancer of zeste homolog 2 (EZH2) in the migration and chemotaxis of human dental tissue-derived mesenchymal stem cells. Methods The expression of EZH2, C-X-C motif chemokine ligand 11 (CXCL11), CXCL16, and CXCR1 in stem cells from the apical papilla (SCAPs) was determined by real-time reverse transcription PCR and western blotting. The effects of EZH2 on the homing of SCAPs and the effects of EZH2-overexpressing SCAP culture supernatant on periodontal ligament stem cells (PDLSCs) were tested by scratch migration assays and transwell chemotaxis assays. Results EZH2 overexpression significantly enhanced the migration and chemotaxis of SCAPs and upregulated the expression of CXCL11, CXCL16, and CXCR1 in SCAPs. EZH2 depletion had the opposite effect, impairing the migration and chemotaxis of SCAPs and downregulating the expression of CXCL11, CXCL16, and CXCR1. The culture supernatant of EZH2-overexpressing SCAPs advanced the migration and chemotaxis of PDLSCs. Conclusions EZH2 evidently promoted the migration and chemotaxis of SCAPs by upregulating the expression of CXCL11, CXCL16, and CXCR1. Moreover, EZH2-overexpressing SCAPs enhanced the homing, migration, and chemotaxis of PDLSCs via paracrine signaling.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81771026]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区药学
最新[2025]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区药学
JCR分区：
出版当年[2018]版：
Q4PHARMACOLOGY & PHARMACYQ4MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q4MEDICINE, RESEARCH & EXPERIMENTALQ4PHARMACOLOGY & PHARMACY
第一作者单位：[1]Department of Stomatology, Beijing Friendship Hospital,Capital Medical University, Beijing, China
通讯作者：
通讯机构：[1]Department of Stomatology, Beijing Friendship Hospital,Capital Medical University, Beijing, China[*1]Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
推荐引用方式(GB/T 7714)：
Ma Huarui,Duan Shaoyu,Yan Fei,et al.Enhancer of zeste homolog 2 enhances the migration and chemotaxis of dental mesenchymal stem cells[J].JOURNAL of INTERNATIONAL MEDICAL RESEARCH.2020,48(1):doi:10.1177/0300060519882149.
APA：
Ma, Huarui,Duan, Shaoyu,Yan, Fei,Yang, Haoqing,Cao, Yangyang...&Gao, Runtao.(2020).Enhancer of zeste homolog 2 enhances the migration and chemotaxis of dental mesenchymal stem cells.JOURNAL of INTERNATIONAL MEDICAL RESEARCH,48,(1)
MLA：
Ma, Huarui,et al."Enhancer of zeste homolog 2 enhances the migration and chemotaxis of dental mesenchymal stem cells".JOURNAL of INTERNATIONAL MEDICAL RESEARCH 48..1(2020)
