单位：[1]Medical and Healthy Analysis Center, Peking University, Beijing 100191, China[2]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China[3]Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing 100029, China[4]Department of Surgical Oncology, Qinghai Provincial People's Hospital, Xining City, Qinghai Province 810007, China
Currently, analyzing intact glycopeptides remains a challengeable task. Considerable progress has been achieved in the knowledge of immunoglobulin G (IgG) glycans in patients with colorectal cancer (CRC), whereas data on IgG Fc N-glycopeptides are scarce in the literature. To fill this gap in knowledge, we developed a rapid and effective method to obtain and analyze IgG Fc N-glycopeptides in the plasma from 46 CRC patients and 67 healthy individuals using chitosan@poly (glycidyl methacrylate) @iminodiacetic acid (CS@PGMA@IDA) nanomaterial in combination with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). A total of 29 N-glycopeptides were detected and analyzed. Compared with healthy individuals, CRC patients had increased levels of N-acteylglucosamine, yet decreased levels of galactosylation, fucosylation and sialylation. Further, a multivariate logistic regression model was developed using the levels of IgG Fc N-glycopeptides to distinguish CRC patients from healthy individuals, and the prediction performance was good, with an average AUC of the ROC curves of 0.893. Significance: In this study, we proposed a strategy for obtaining and analyzing IgG glycopeptides using CS@PGMA@IDA nanomaterial in combination with MALDI-TOF-MS. Using this strategy, IgG Fc N-glycopeptides were analyzed in the plasma of CRC patients, and our findings indicated that glycosylation levels in the IgG Fc region were closely related to CRC. By using the IgG N-glycopeptide enrichment method and screening model designed in this study, early large-scale colorectal cancer screening can be implemented easily and fast.
基金：
Seeding Grant for Medicine and Information Sciences of Peking University [2014 -MI-21]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21572010, 21772005]; Qinghai Department of Science Technology [2015-ZJ-742]
第一作者单位：[1]Medical and Healthy Analysis Center, Peking University, Beijing 100191, China[2]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
共同第一作者：
通讯作者：
通讯机构：[1]Medical and Healthy Analysis Center, Peking University, Beijing 100191, China[2]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China[*1]Medical and Healthy Analysis Center, Beijing Key Laboratory of Tumor Systems Biology, Peking University, Beijing 100191, China[*2]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
推荐引用方式(GB/T 7714)：
Zou Yang,Hu Jianxing,Jie Jianzheng,et al.Comprehensive analysis of human IgG Fc N-glycopeptides and construction of a screening model for colorectal cancer[J].JOURNAL of PROTEOMICS.2020,213:doi:10.1016/j.jprot.2019.103616.
APA：
Zou, Yang,Hu, Jianxing,Jie, Jianzheng,Lai, Junyong,Li, Mingna...&Zou, Xiajuan.(2020).Comprehensive analysis of human IgG Fc N-glycopeptides and construction of a screening model for colorectal cancer.JOURNAL of PROTEOMICS,213,
MLA：
Zou, Yang,et al."Comprehensive analysis of human IgG Fc N-glycopeptides and construction of a screening model for colorectal cancer".JOURNAL of PROTEOMICS 213.(2020)
