单位：[a]Department of Cardiac Surgery, The First Hospital of Tsinghua University,Beijing, China[b]Department of Anesthesiology, Beijing Anzhen Hospital, BeijingInstitute of Heart, Lung and Blood Vessel Diseases, Capital Medical University,Beijing, China首都医科大学附属安贞医院[c]Department of Anesthesiology, Beijing Friendship Hospital, CapitalMedical University, Beijing, China临床科室麻醉科麻醉科首都医科大学附属北京友谊医院[d]Department of Epidemiology andHealth Statistics, School of Public Health, Capital Medical University, Beijing,China
Objectives: Delayed paraplegia developed postoperatively after thoracoabdominal aneurysm surgery is primarily associated with spinal cord ischemia/reperfusion injury. Our previous study suggested that spinal cord stimulation postconditioning protected the spinal cord from ischemia/reperfusion injury through microglia inhibition. In this study, we further investigated whether alpha 7 nicotinic acetylcholine receptors were involved in the neuroprotective mechanism of spinal cord stimulation. Methods: Rabbits were randomly assigned to sham, control, 2 Hz, alpha-bungarotoxin, and 2 Hz-alpha-bungarotoxin groups (n = 24/group). Transient spinal cord ischemia was performed on all rabbits except rabbits in the sham group. Rabbits in the control group received no further intervention, rabbits in the 2 Hz group were given 2 Hz spinal cord stimulation, rabbits in the alpha-bungarotoxin group received prescribed intrathecal alpha-bungarotoxin (alpha-bungarotoxin, a specific alpha 7 nicotinic acetylcholine receptor antagonist) injections, and rabbits in the 2 Hz-alpha-bungarotoxin group received both alpha-bungarotoxin injections and 2 Hz spinal cord stimulation. Hind-limb neurologic function was assessed, and spinal cord histologic examination, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining, and microglia staining were performed at 8 hours, 1 day, 3 days, and 7 days of reperfusion. Results: Rabbits in the 2 Hz group had significantly better neurologic functions, more alpha-motor neurons, and lower terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive neuron rates and microglia area/anterior horn area ratios (microglia area ratios) than the control group. The neurologic functions of the alpha-bungarotoxin group were significantly worse than those of the control group, whereas other results were not significantly different from the control group. The results of the 2 Hz-alpha-bungarotoxin group were insignificant to the control group except for the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive neuron rates, which were significantly lower than in the control group. Conclusions: The neuroprotective effects of spinal cord stimulation postconditioning against spinal cord ischemia/reperfusion injury were partially mediated by activating alpha 7 nicotinic acetylcholine receptors.
基金：
Beijing Natural Science FoundationBeijing Natural Science Foundation [7152046]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|2 区医学
小类|1 区外科2 区心脏和心血管系统2 区呼吸系统
最新[2025]版：
大类|2 区医学
小类|1 区外科2 区心脏和心血管系统2 区呼吸系统
JCR分区：
出版当年[2018]版：
Q1CARDIAC & CARDIOVASCULAR SYSTEMSQ1SURGERYQ1RESPIRATORY SYSTEM
第一作者单位：[a]Department of Cardiac Surgery, The First Hospital of Tsinghua University,Beijing, China
通讯作者：
通讯机构：[b]Department of Anesthesiology, Beijing Anzhen Hospital, BeijingInstitute of Heart, Lung and Blood Vessel Diseases, Capital Medical University,Beijing, China[c]Department of Anesthesiology, Beijing Friendship Hospital, CapitalMedical University, Beijing, China[d]Department of Epidemiology andHealth Statistics, School of Public Health, Capital Medical University, Beijing,China[*1]Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China[*2]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China[*3]Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University, Beijing, China
推荐引用方式(GB/T 7714)：
Huixian Li,Xiuhua Dong,Weiping Cheng,et al.Neuroprotective mechanism involved in spinal cord stimulation postconditioning[J].JOURNAL of THORACIC and CARDIOVASCULAR SURGERY.2020,159(3):813-+.doi:10.1016/j.jtcvs.2019.03.048.
APA：
Huixian Li,Xiuhua Dong,Weiping Cheng,Mu Jin&Deqiang Zheng.(2020).Neuroprotective mechanism involved in spinal cord stimulation postconditioning.JOURNAL of THORACIC and CARDIOVASCULAR SURGERY,159,(3)
MLA：
Huixian Li,et al."Neuroprotective mechanism involved in spinal cord stimulation postconditioning".JOURNAL of THORACIC and CARDIOVASCULAR SURGERY 159..3(2020):813-+
医学