单位：[1]Department of Otolaryngology – Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China临床科室耳鼻咽喉头颈外科耳鼻咽喉头颈外科首都医科大学附属北京友谊医院[2]Department of Otolaryngology – Head and Neck Surgery, Eye Hospital China Academy of Chinese Medical Science, Beijing, China
To examine whether exposure to sodium salicylate disrupts expression of vesicular glutamate transporter 3 (VGLUT3) and whether the alteration in expression corresponds to increased risk for tinnitus. Rats were treated with saline (control) or sodium salicylate (treated) Rats were examined for tinnitus by monitoring gap-pre-pulse inhibition of the acoustic startle reflex (GPIAS). Auditory brainstem response (ABR) was applied to evaluate hearing function after treatment. Rats were sacrificed after injection to obtain the cochlea, cochlear nucleus (CN), and inferior colliculus (IC) for examination of VGLUT3 expression. No significant differences in hearing thresholds between groups were identified (p>0.05). Tinnitus in sodium salicylate-treated rats was confirmed by GPIAS. VGLUT3 encoded by solute carrier family 17 members 8 (SLC17a8) expression was significantly increased in inner hair cells (IHCs) of the cochlea in treated animals, compared with controls (p<0.01). No significant differences in VGLUT3 expression between groups were found for the cochlear nucleus (CN) or IC (p>0.05). Exposure to sodium salicylate may disrupt SLC17a8 expression in IHCs, leading to alterations that correspond to tinnitus in rats. However, the CN and IC are unaffected by exposure to sodium salicylate, suggesting that enhancement of VGLUT3 expression in IHCs may contribute to the pathogenesis of tinnitus.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81500789, 81700917]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7174291]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2016M601067]
第一作者单位：[1]Department of Otolaryngology – Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China[2]Department of Otolaryngology – Head and Neck Surgery, Eye Hospital China Academy of Chinese Medical Science, Beijing, China
通讯作者：
通讯机构：[1]Department of Otolaryngology – Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China[*1]Department of Otolaryngology – Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong An, Beijing, 100050, China
推荐引用方式(GB/T 7714)：
Zhang Wei,Peng Zhe,Yu Shukui,et al.Exposure to Sodium Salicylate Disrupts VGLUT3 Expression in Cochlear Inner Hair Cells and Contributes to Tinnitus[J].PHYSIOLOGICAL RESEARCH.2020,69(1):181-190.doi:10.33549/physiolres.934180.
APA：
Zhang, Wei,Peng, Zhe,Yu, Shukui,Song, Qing-Ling,Qu, Teng-Fei...&Gong, Shu-Sheng.(2020).Exposure to Sodium Salicylate Disrupts VGLUT3 Expression in Cochlear Inner Hair Cells and Contributes to Tinnitus.PHYSIOLOGICAL RESEARCH,69,(1)
MLA：
Zhang, Wei,et al."Exposure to Sodium Salicylate Disrupts VGLUT3 Expression in Cochlear Inner Hair Cells and Contributes to Tinnitus".PHYSIOLOGICAL RESEARCH 69..1(2020):181-190
