单位：[1]Beijing Key Laboratory of Respiratoryand Pulmonary Circulation Disorders,Department of Respiratory Medicine,Capital Medical University, Beijing,People’s Republic of China北京朝阳医院[2]Departmentof Respiratory Medicine, ZhengzhouCentral Hospital, Zhengzhou, People’sRepublic of China[3]Department ofRadiology, China-Japan FriendshipHospital, Beijing, People’s Republic ofChina[4]Beijing Key Laboratory ofRespiratory and Pulmonary CirculationDisorders, Department of MedicalResearch, Beijing Chao-Yang Hospital,Capital Medical University, Beijing,People’s Republic of China北京朝阳医院[5]Departmentof Pulmonary and Critical Care Medicine,Center of Respiratory Medicine, China-Japan Friendship Hospital,NationalClinical Research Center for RespiratoryDiseases,Capital Medical University,Beijing, People’s Republic of China
Background: Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fibrotic interstitial pneumonia. And, oxidation/antioxidant imbalance plays an important role in the progress of IPF. Fullerene is considered to be a novel "structural" antioxidant. This study aimed to explore if water-soluble C-60 (C-60(OH)(22)) can exhibit antifibrotic activity in its antioxidant role. Methods: Healthy C57BL/6J mice were randomly grouped and induced pulmonary fibrosis by intratracheal injection of bleomycin. Results: The survival rate of mice was observed and found that 10mg/kg was the optimal dose of water-soluble C-60 for pulmonary fibrosis. We observed that water-soluble C-60 can alleviate the severity of pulmonary fibrosis by observing the chest computed tomography, pulmonary pathology, and content of collagen, alpha smooth muscle actin and fibronectin in lung. Compared with bleomycin group, ROS, the content of TNF-alpha in BALF, and the number of fibroblasts was significantly decreased and the number of type II alveolar epithelial cells was increased after treatment with C-60. Conclusion: Therefore, thanks to its powerful antioxidant action, water-soluble C-60 can reduce the severity of pulmonary fibrosis induced by bleomycin in mice.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81430001, 81470258, 81871328]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7182149]
第一作者单位：[1]Beijing Key Laboratory of Respiratoryand Pulmonary Circulation Disorders,Department of Respiratory Medicine,Capital Medical University, Beijing,People’s Republic of China[2]Departmentof Respiratory Medicine, ZhengzhouCentral Hospital, Zhengzhou, People’sRepublic of China
共同第一作者：
通讯作者：
通讯机构：[5]Departmentof Pulmonary and Critical Care Medicine,Center of Respiratory Medicine, China-Japan Friendship Hospital,NationalClinical Research Center for RespiratoryDiseases,Capital Medical University,Beijing, People’s Republic of China[*1]Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, People’s Republic of China
推荐引用方式(GB/T 7714)：
Dong Run,Liu Min,Huang Xiao-Xi,et al.Water-Soluble C-60 Protects Against Bleomycin-Induced Pulmonary Fibrosis in Mice[J].INTERNATIONAL JOURNAL of NANOMEDICINE.2020,15:2269-2276.doi:10.2147/IJN.S214056.
APA：
Dong, Run,Liu, Min,Huang, Xiao-Xi,Liu, Zheng,Jiang, Ding-Yuan...&Dai, Hua-Ping.(2020).Water-Soluble C-60 Protects Against Bleomycin-Induced Pulmonary Fibrosis in Mice.INTERNATIONAL JOURNAL of NANOMEDICINE,15,
MLA：
Dong, Run,et al."Water-Soluble C-60 Protects Against Bleomycin-Induced Pulmonary Fibrosis in Mice".INTERNATIONAL JOURNAL of NANOMEDICINE 15.(2020):2269-2276
