单位：[1]Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China.[2]School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, China.[3]Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing, China.[4]The Second Clinical College of Guangzhou, University of Chinese Medicine, Guangzhou, China.广东省中医院[5]Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.[6]Academy of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Background: Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovitis. Pien Tze Huang (PZH) is a Chinese patent medicine with anti-inflammatory and immunomodulatory effects. However, whether PZH could be used in RA therapy is still unknown. Therefore, this study aimed to explore the therapeutic effect and the potential mechanism of PZH on collagen-induced arthritis (CIA) mice. Methods: Male DBA/1J mice were used to establish an animal model of CIA and then treated with different doses of PZH for 4 weeks. The therapeutic effect of PZH on CIA mice was evaluated by arthritis score, pathological staining, and detecting the levels of inflammatory factors in serum and joints. To investigate its possible mechanism, the activity of NF-kappa B signaling pathway, NLRP3 inflammasome and the level of A20 were detected. Results: The results showed that PZH could alleviate the erythema and swelling of hind paws of CIA mice, improve the pathological conditions of joint and decrease the production of IL-1 beta, IL-6 and IL-17 in serum and joints. Furthermore, PZH could significantly inhibit the activity of NF-kappa B signaling pathway and NLRP3 inflammasome in the ankle joint of CIA mice compared with the model group. It also increased the level of A20 in the ankle joint of CIA mice. Conclusion: This study indicated that PZH could alleviate the joint inflammation of CIA mice, and the mechanism might be related to the regulation of NF-kappa B signaling pathway and NLRP3 inflammasome.
基金：
National Key R&D Program of China [2018YFC1705205]
第一作者单位：[1]Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China.[2]School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, China.
通讯作者：
通讯机构：[5]Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.[6]Academy of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
推荐引用方式(GB/T 7714)：
YongQi Deng,Hui Luo,Jun Shu,et al.Pien Tze Huang alleviate the joint inflammation in collagen-induced arthritis mice[J].CHINESE MEDICINE.2020,15(1):doi:10.1186/s13020-020-00311-3.
APA：
YongQi Deng,Hui Luo,Jun Shu,Haiyang Shu,Cheng Lu...&Aiping Lu.(2020).Pien Tze Huang alleviate the joint inflammation in collagen-induced arthritis mice.CHINESE MEDICINE,15,(1)
MLA：
YongQi Deng,et al."Pien Tze Huang alleviate the joint inflammation in collagen-induced arthritis mice".CHINESE MEDICINE 15..1(2020)
