Directional axonal regrowth induced by an aligned fibrin nanofiber hydrogel contributes to improved motor function recovery in canine L2 spinal cord injury
单位：[1]State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, 100084 Beijing, China[2]School of Materials Science and Engineering, University of Science and Technology Beijing, 100083 Beijing, China[3]Center for Biomedical Imaging Research, Tsinghua University, 100084 Beijing, China[4]Department of Radiology, China–Japan Friendship Hospital, 100029 Beijing, China[5]Department of Orthopedics, Dongzhimen Hospital, 100007 Beijing, China[6]Beijing Tsinghua Chang Gung Hospital, School of Clinical Medicine, Tsinghua University, 102218 Beijing, China
Spinal cord injuries (SCI) normally disrupt the long axonal tracts of the spinal cord and cause permanent neurological deficits, for which there is currently a lack of effective therapeutic methods. Biomaterial-based regenerative medicine is a pivotal strategy to induce axonal regeneration through delivery of biophysical and/or biochemical regulatory cues by biomaterials. We previously fabricated a hierarchically aligned fibrin hydrogel (AFG) that could promote neurogenic differentiation of stem cells in vitro and has been successfully applied for peripheral nerve and spinal cord regeneration in rats. In this study, AFG was used to repair a canine lumbar segment 2 hemisection spinal cord injury, and the consistency of histological, imageological and behavioral results was compared. AFG was used to construct an aligned fiber bridge that supported cell adhesion in vitro and rapidly facilitated tissue invasion along the long axis of fibers in vivo, Moreover, in vivo results demonstrated regrowth of axons in an oriented pattern connecting the rostral and caudal stumps. Consistent results were confirmed by diffusion tensor imaging, which allowed successful tracing of reconnected nerve fibers across the defect. As a result, directional axonal regrowth contributed to significantly improved recovery of motor functional behavior of SCI canines with AFG implantation. Our results suggest that AFG has great promise for rapidly directing axonal regrowth for nerve regeneration.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31771056]; National Key R&D Program of China [2018YFB0704304-1]; Tsinghua University Initiative Scientific Research Program [20161080091]
第一作者单位：[1]State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, 100084 Beijing, China
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推荐引用方式(GB/T 7714)：
Zheng Cao,Shenglian Yao,Yuhui Xiong,et al.Directional axonal regrowth induced by an aligned fibrin nanofiber hydrogel contributes to improved motor function recovery in canine L2 spinal cord injury[J].JOURNAL of MATERIALS SCIENCE-MATERIALS in MEDICINE.2020,31(5):doi:10.1007/s10856-020-06375-9.
APA：
Zheng Cao,Shenglian Yao,Yuhui Xiong,Zhenxia Zhang,Yongdong Yang...&Xiumei Wang.(2020).Directional axonal regrowth induced by an aligned fibrin nanofiber hydrogel contributes to improved motor function recovery in canine L2 spinal cord injury.JOURNAL of MATERIALS SCIENCE-MATERIALS in MEDICINE,31,(5)
MLA：
Zheng Cao,et al."Directional axonal regrowth induced by an aligned fibrin nanofiber hydrogel contributes to improved motor function recovery in canine L2 spinal cord injury".JOURNAL of MATERIALS SCIENCE-MATERIALS in MEDICINE 31..5(2020)
