Persistent proliferation of keratinocytes and prolonged expression of pronociceptive inflammatory mediators might be associated with the postoperative pain in KK mice
单位：[1]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China临床科室麻醉科麻醉科首都医科大学附属北京友谊医院[2]Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China北京朝阳医院
Epidermal keratinocytes play a vital role in restoration of the intact skin barrier during wound healing. The negative effect of hyperglycemia may prolong the wound healing process. Epidermal keratinocytes have been demonstrated to modulate and directly initiate nociceptive responses in rat models of fractures and chemotherapy-induced neuropathic pain. However, it is unclear whether epidermal keratinocytes are involved in the development and maintenance of incisional pain in nondiabetic or diabetic animals. In the current study, using behavioral tests and immunohistochemistry, we investigated the differential keratinocytes proliferation and expression of pronociceptive inflammatory mediators in keratinocytes in C57BL/6J mice and diabetic KK mice. Our data showed that plantar incision induced postoperative pain hypersensitivity in both C57BL/6J mice and KK mice, while the duration of postoperative pain hypersensitivity in KK mice was longer than that in C57BL/6J mice. Moreover, plantar incision induced the keratinocytes proliferation and expression of IL-1 beta and TNF-alpha in keratinocytes in both C57BL/6J mice and KK mice. Interestingly, compared to C57BL/6J mice, the slower and more persistent proliferation of keratinocytes and expression of IL-1 beta and TNF-alpha in keratinocytes were observed in KK mice. Together, our study suggested that plantar incision may induce the differential keratinocytes proliferation and expression of IL-1 beta and TNF-alpha in kertinocytes in diabetic and nondiabetic animals, which might be associated with the development and maintenance differences in diabetic and nondiabetic postoperative pain.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81571065, 81771181]; Natural Science Foundation of BeijingBeijing Natural Science Foundation [7202053]; Beijing Hospitals Authority Youth Program [QML20180105]; Scientific Research Common Program of Beijing Municipal Commission of EducationBeijing Municipal Commission of Education [KM201910025018]
第一作者单位：[1]Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[2]Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China[*1]Department of Anesthesiology Beijing Chaoyang Hospital, Capital Medical University, No. 8, Gongtinan Road, Chaoyang District, Beijing 100020, China
推荐引用方式(GB/T 7714)：
Guo Ruijuan,Hao Junqiang,Ma Danxu,et al.Persistent proliferation of keratinocytes and prolonged expression of pronociceptive inflammatory mediators might be associated with the postoperative pain in KK mice[J].MOLECULAR PAIN.2020,16:doi:10.1177/1744806920927284.
APA：
Guo, Ruijuan,Hao, Junqiang,Ma, Danxu,Li, Huili,Liao, Kaihua&Wang, Yun.(2020).Persistent proliferation of keratinocytes and prolonged expression of pronociceptive inflammatory mediators might be associated with the postoperative pain in KK mice.MOLECULAR PAIN,16,
MLA：
Guo, Ruijuan,et al."Persistent proliferation of keratinocytes and prolonged expression of pronociceptive inflammatory mediators might be associated with the postoperative pain in KK mice".MOLECULAR PAIN 16.(2020)
