单位：[1]Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China临床科室肿瘤中心肿瘤内科首都医科大学附属北京友谊医院[2]Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China[3]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing 100050, China临床科室国家中心消化分中心消化内科首都医科大学附属北京友谊医院
Gastric cancer (GC) is one of the most common and fatal malignancies worldwide. MicroRNAs (miRNAs) play a critical role in tumor initiation, proliferation, and metastasis of gastric cancer. miR193b has been identified as a tumor suppressor in a variety of tumor types; however, its role in gastric cancer is yet to be determined. Here, we found a significant downregulation of miR193b expression in both human gastric cancer tissues (p<0.05) and human gastric cancer cell lines (p<0.01). Furthermore, the expression level of miR193b correlated with the tumor type, tumor size, and clinical stage (p<0.05). In vitro, miR193b overexpression inhibited cell survival and induced apoptosis in GC cell lines, indicating that miR193b plays a role in the development of gastric cancer. KRAS was verified as the target of miR193b, and KRAS overexpression attenuated miR193b-induced apoptosis (p<0.05). Moreover, we found that the Akt pathway negatively regulated miR193b, also affecting apoptosis. Further analyses indicated that PIK3CA mutation and KRAS amplification are two mutually exclusive pathways (p<0.01), and we hypothesize that both two pathways could result in the carcinogenic overactivation of KRAS. Thus, our results suggest that the Akt-miR193b-KRAS axis may act as a mechanism affecting apoptosis in gastric cancer cells.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670474]; Natural Science Foundation of BeijingBeijing Natural Science Foundation [7152043]; Digestive Medical Coordinated Development Center of Beijing Hospitals Authority [XXT01]; China Population Publicity and Education Center Research and Promotion Project [2017-A001]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2018]版：
Q3MEDICINE, RESEARCH & EXPERIMENTALQ3BIOTECHNOLOGY & APPLIED MICROBIOLOGY
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
通讯作者：
推荐引用方式(GB/T 7714)：
Tian Ruyue,Jiang Hailun,Shao Linlin,et al.miR193b Promotes Apoptosis of Gastric Cancer Cells via Directly Mediating the Akt Pathway[J].BIOMED RESEARCH INTERNATIONAL.2020,2020:doi:10.1155/2020/2863236.
APA：
Tian, Ruyue,Jiang, Hailun,Shao, Linlin,Yu, Yang,Guo, Qingdong...&Guo, Shuilong.(2020).miR193b Promotes Apoptosis of Gastric Cancer Cells via Directly Mediating the Akt Pathway.BIOMED RESEARCH INTERNATIONAL,2020,
MLA：
Tian, Ruyue,et al."miR193b Promotes Apoptosis of Gastric Cancer Cells via Directly Mediating the Akt Pathway".BIOMED RESEARCH INTERNATIONAL 2020.(2020)
