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DIELECTROPHORETIC CELL SEPARATION USING CONDUCTING SILVER PDMS MICROELECTRODES FEATURING NON-UNIFORM SIDEWALL PROFILE

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单位: [1]College of Information Science and Technology, Beijing University of Chemical Technology, P. R.of CHINA [2]Department of Clinical Laboratory, China-Japan Friendship Hospital, P. R. of CHINA [3]Beijing Advanced Innovation Center for Soft Matter Science and Engineering, P. R. of CHINA
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This work for the first time uses silver PDMS (AgPDMS) composites to construct 3D microelectrodes featuring non-uniform sidewall profile. Such design enables an innovative dielectrophoretic (DEP) cell sorting device with electrodes and fluidic structures being molded simultaneously and completely made of AgPDMS. The interdigitated electrode digits of the device exhibit inhomogeneous two-layer structure, which projects highly non-uniform electric field that separates cells not only laterally but also vertically along the channel depth. Meanwhile, such design leads to effective focusing of cells under nDEP repulsion. The device has been showcased here for continuous-flow separation of 15 mu m from 2 mu m beads, as well as separation of human erythrocytes from similar sized 7 mu m beads with simultaneous focusing of the particles under nDEP.

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第一作者单位: [1]College of Information Science and Technology, Beijing University of Chemical Technology, P. R.of CHINA
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