单位：[1]Department of Respiratory and Critical Care Medicine, National Center of Gerontology, Beijing Hospital, Beijing, China[2]Clinical Biobank, National Center of Gerontology, Beijing Hospital, Beijing, China[3]Department of Laboratory Medicine, Beijing Hospital, Beijing, China[4]National Clinical Research Center for Respiratory Diseases, Center for Respiratory Diseases, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, China[5]Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, China[6]Department of Respiratory and Critical Care Medicine, Bengbu University Affiliated Hospital, Bengbu, China[7]Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University and Beijing Institute of Respiratory Medicine, Beijing, China北京朝阳医院[8]The Key Laboratory of Geriatrics, National Center of Gerontology, Beijing Hospital, Beijing, China[9]Annoroad Gene Technology (Beijing) Co., Ltd., Beijing, China[10]National Clinical Research Center for Respiratory Diseases, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China
Lung microbiome ecosystem homeostasis in idiopathic pulmonary fibrosis (IPF) remains uncharacterized. The aims of this study were to identify unique microbial signatures of the lung microbiome and analyze microbial gene function in IPF patients. DNA isolated from BALF samples was obtained for high-throughput gene sequencing. Microbial metagenomic data were used for principal component analysis (PCA) and analyzed at different taxonomic levels. Shotgun metagenomic data were annotated using the KEGG database and were analyzed for functional and metabolic pathways. In this study, 17 IPF patients and 38 healthy subjects (smokers and non-smokers) were recruited. For the PCA, the first and the second principal component explained 16.3 and 13.4% of the overall variability, respectively. The beta diversity of microbiome was reduced in the IPF group. Signature of IPF's microbes was enriched of Streptococcus, Pseudobutyrivibrio, and Anaerorhabdus. The translocation of lung microbiome was shown that 32.84% of them were from oral. After analysis of gene function, ABC transporter systems, biofilm formation, and two-component regulatory system were enriched in IPF patients' microbiome. Here we shown the microbiology characteristics in IPF patients. The microbiome may participate in altering internal conditions and involving in generating antibiotic resistance in IPF patients.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81400037]; Public Health Special Research of the Ministry of Health of the People's Republic of China [2015SQ00301, 201302017]; Beijing Hospital Nova Project [BJ-2016-039]
第一作者单位：[1]Department of Respiratory and Critical Care Medicine, National Center of Gerontology, Beijing Hospital, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[4]National Clinical Research Center for Respiratory Diseases, Center for Respiratory Diseases, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, China[5]Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, China[10]National Clinical Research Center for Respiratory Diseases, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China
推荐引用方式(GB/T 7714)：
Tong Xunliang,Su Fei,Xu Xiaomao,et al.Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients[J].FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY.2019,9:doi:10.3389/fcimb.2019.00149.
APA：
Tong, Xunliang,Su, Fei,Xu, Xiaomao,Xu, Hongtao,Yang, Ting...&Wang, Chen.(2019).Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients.FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY,9,
MLA：
Tong, Xunliang,et al."Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients".FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY 9.(2019)
