单位：[1]Department of Nephrology, Faculty of Kidney Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, PR China临床科室肾脏内科肾脏内科首都医科大学附属北京友谊医院
Background: Cardiovascular disease is the leading cause of death in patients with chronic kidney disease, so there is an urgent need to identify therapeutic targets to control the progression of cardiovascular disease. Apoptosis of aortic smooth muscle cells can promote cardiovascular disease, but the role of parathyroid hormone (PTH) and sirtuin 1 in the pathophysiology of apoptosis is still unclear. Methods: Cultured human aortic smooth muscle cells (HASMCs) were stimulated with 10(-6), 10(-8), or 10(-10) mol/L PTH for different days, apoptosis was measured by flow cytometry and sirtuin 1 and Bcl-2 protein levels in cell extracts were analyzed by western blotting. HASMCs were stimulated with PTH (10(-8) mol/L) and 50 or 100 mu mol/L RES for 3 d, apoptosis was measured by flow cytometry and sirtuin 1 and Bcl-2 protein levels in cell extracts were analyzed by western blotting. Results: We found that PTH decreased the expression of sirtuin 1 and Bcl-2, inducing apoptosis (p<.05). Resveratrol (RES), a sirtuin 1 agonist, inhibited PTH-induced apoptosis and restored Bcl-2 expression (p<.05). Conclusions: PTH induces apoptosis in HASMCs. Resveratrol inhibits PTH-induced apoptosis in HASMCs.
基金：
Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201824]
第一作者单位：[1]Department of Nephrology, Faculty of Kidney Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, PR China
通讯作者：
通讯机构：[1]Department of Nephrology, Faculty of Kidney Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, PR China[*1]Department of Nephrology, Faculty of Kidney Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, PR China
推荐引用方式(GB/T 7714)：
Liu Yingjie,Wu Yiru,Diao Zongli,et al.Resveratrol inhibits parathyroid hormone-induced apoptosis in human aortic smooth muscle cells by upregulating sirtuin 1[J].RENAL FAILURE.2019,41(1):401-407.doi:10.1080/0886022X.2019.1605296.
APA：
Liu, Yingjie,Wu, Yiru,Diao, Zongli,Guo, Weikang&Liu, Wenhu.(2019).Resveratrol inhibits parathyroid hormone-induced apoptosis in human aortic smooth muscle cells by upregulating sirtuin 1.RENAL FAILURE,41,(1)
MLA：
Liu, Yingjie,et al."Resveratrol inhibits parathyroid hormone-induced apoptosis in human aortic smooth muscle cells by upregulating sirtuin 1".RENAL FAILURE 41..1(2019):401-407
