Purpose: To reduce the miss diagnosis under negative result of magnetic resonance cholangiopancreatography (MRCP), a correlation analysis between biochemical indicators and the common bile duct (CBD) stones was performed in this study. Method: A total of 514 patients following the Laparoscopic cholecystectomy with the CBD exploration were enrolled. The biochemical indicators including gamma-glutamyltransferase (GGT) and common bile duct diameter (CBDD), as well as imagological examination results including MRCP were evaluated to reveal the correlations with CBD stone using receiver operating characteristic (ROC) curve and logistic regression analyses. Result: MRCP and CBDD were two outstanding indexes related with the CBD stone. ROC analysis showed that ALP was the most outstanding index for CBD stone patients with negative MRCP, followed by direct bilirubin (DB) and GGT. Meanwhile, ROC analysis and logistic regression analyses showed that GGT was the most outstanding index for CBD stone patients with the negative MRCP combined to normal width CBDD (CBDD <0.8 cm), followed by alkaline phosphatase (ALP) and DB. Conclusion: ALP, DB and GGT might be potential predictors for CBD stone patients when MRCP is negative. If the result of MRCP was negative, especially combined with CBDD <0.8 cm, a further GGT investigation was suggested to determine whether the LCBDE should be performed. (C) 2019 Elsevier B.V. All rights reserved.