This study had three objectives: (1) to investigate healthcare professionals' knowledge, attitude, and practice toward clinically applying graduated compression stockings, (2) to analyze the influencing factors, and (3) to provide data to support departments that develop health policies. A self-administered web-based survey was completed by 1,444 healthcare professionals, including physicians and nurses from 15tertiary hospitals and four secondary hospitals from 10 provinces in China. Reliability analysis and exploratory factor analysis were used to evaluate the researcher-designed questionnaire's reliability and validity. The formal questionnaire, which included demographic data (eight items), knowledge (ten items), attitudes (four items), and clinical practice patterns (six items), was distributed among healthcare professionals. The relationships and mechanisms among the variables were explored using descriptive statistical analysis, Pearson's correlation coefficients, and multiple linear regression analysis. Of the 1,444 respondents, 31.2% had good knowledge of clinically applying graduated compression stockings, 83.5% had a positive attitude toward clinically applying them, and 30.4% of respondents exhibited normative behavior when applying them. The multiple linear regression analysis indicated that training was an important factor influencing the knowledge, attitude, and practice toward clinically applying graduated compression stockings. The healthcare professionals' attitude toward clinically applying graduated compression stockings was positive, but the related knowledge was poor, and the code of behavior was deficient. Medical institutions should improve training for clinically applying graduated compression stockings among healthcare professionals and standardize the use of graduated compression stockings to facilitate the prevention of hospital-acquired venous thromboembolism.