Objective: To examine the effect of connective tissue growth factor (CTGF)-mediated ERK signaling pathway on the inflammatory response and intestinal flora in ulcerative colitis (UC). Methods: CTGF expression was determined through immunohistochemistry in UC and colon polyp patients. Dextran sulfate sodium (DSS) was used to construct UC models. Wild-type (WT) and CTGF(-/-) deficient (CTGF(-/-)) mice were randomly divided into WT/CTGF(-/-) + saline, WT/CTGF(-/-) + DSS, and WT/CTGF(-/-) + DSS + U0126 (ERK pathway inhibitor) groups. HE staining was conducted to observe the pathological changes in intestinal mucosa. The quantity of intestinal flora was tested in the feces. ELISA, qRT-PCR, and Western blotting were used to detect related-molecules expressions. Results: CTGF was up-regulated in the intestinal mucosa of UC patients in relation to the severity and grade. Moreover, UC patients showed enhanced the expressions of p-ERK/ERK and pro-inflammatory factors (IL-1 beta, IL-6, TNF-alpha, MPO), increased the quantity of Bacteriodes fragilis (B. fragilis) and Escherichia coll. (E. coil), and decreased Bifidobacterium and Lactobacillus. CTGF and pERK/ERK expressions were increased in DSS-induced WT mice, but the pERK expression was lower in CTGF(-/-) + DSS group than that in the WT + DSS group. U0126 decreased the expressions of pro-inflammatory factors and improved the intestinal flora in WT mice induced with DSS. No significant differences were found in the above indexes between CTGF(-/-) + DSS group and WT + DSS + U0126 group. Conclusion: Inhibiting CTGF could improve inflammatory response and intestinal flora to partially reverse DSS induced UC via blocking ERK signaling pathway.
基金：
China-Japan Friendship Hospital Youth Science and Technology Excellence Project [2015-QNYC-B-02]; Scientific and Technological Project for Overseas Chinese Students of The Ministry of Human Resources and Social Security [2015-042]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区医学
小类|3 区医学：研究与实验3 区药学
最新[2025]版：
大类|2 区医学
小类|2 区医学：研究与实验2 区药学
JCR分区：
出版当年[2017]版：
Q2PHARMACOLOGY & PHARMACYQ2MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ1PHARMACOLOGY & PHARMACY
第一作者单位：[1]Department of Gastroenterology, China-Japan Friendship Hospital, Beijing, 100029, PR China
通讯作者：
通讯机构：[1]Department of Gastroenterology, China-Japan Friendship Hospital, Beijing, 100029, PR China[*1]Department of Gastroenterology, China-Japan Friendship Hospital, No. 2, Yinghua East Street, Chaoyang District, Beijing, 100029, PR China
推荐引用方式(GB/T 7714)：
Song Zhen-Mei,Liu Fang,Chen Yan-Ming,et al.CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis[J].BIOMEDICINE & PHARMACOTHERAPY.2019,111:1429-1437.doi:10.1016/j.biopha.2018.12.063.
APA：
Song, Zhen-Mei,Liu, Fang,Chen, Yan-Ming,Liu, Yi-Jing,Wang, Xiao-Di&Du, Shi-Yu.(2019).CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis.BIOMEDICINE & PHARMACOTHERAPY,111,
MLA：
Song, Zhen-Mei,et al."CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis".BIOMEDICINE & PHARMACOTHERAPY 111.(2019):1429-1437
