单位：[1]Department of Pharmacy, Liuzhou Traditional Chinese Medical Hospital, Guangxi, People's Republic of China[2]Department of Pharmacy, Beijing Friendship Hospital Pinggu Campus, Capital Medical University, Beijing, People's Republic of China医技科室药学部首都医科大学附属北京友谊医院[3]College of Pharmacy and Health Sciences, Western New England University, Springfield, Massachusetts, USA[4]Department of Pharmacy, The General Hospital of the Chinese People's Armed Police Forces, Beijing, People's Republic of China[5]Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China首都医科大学附属天坛医院
Valproic acid (VPA) pharmacokinetics is highly variable and monitoring of blood levels is necessary to determine its appropriate dosage. This study aimed to establish and validate a novel derivatization method for the determination of VPA. The method was based on the catalytic effect of tetramethylammonium hydroxide using 2,4 '-dibromoacetophenone as a derivatization reagent. After derivatization, samples were injected into the HPLC system for analysis. The method showed a good linearity in the range of 1.0-200.7 mu g mL(-1), and the limit of quantification was 1 mu g mL(-1). All values of the accuracy and relative standard deviations were acceptable for the analyses of biological samples. The recoveries were in the range from 91.6 to 97.4% for VPA with RSD <3.9%. A novel and high conversion-rate derivatization method has been developed and validated for the determination of VPA in human serum. It can be applied to the analysis of VPA in clinic serum samples.
基金：
Beijing Municipal Administration of Hospitals' Youth Programme [QML20170506]; Young Scientist Program [YSP201703]; Beijng Municipal Administration of Hospitals' Sailing Programme [ZYLX201827]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|4 区生物
小类|4 区生化研究方法4 区生化与分子生物学4 区分析化学4 区药学
最新[2025]版：
大类|4 区医学
小类|4 区生化研究方法4 区生化与分子生物学4 区分析化学4 区药学
JCR分区：
出版当年[2017]版：
Q3CHEMISTRY, ANALYTICALQ3BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGYQ4PHARMACOLOGY & PHARMACY
最新[2023]版：
Q3CHEMISTRY, ANALYTICALQ3PHARMACOLOGY & PHARMACYQ4BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGY
第一作者单位：[1]Department of Pharmacy, Liuzhou Traditional Chinese Medical Hospital, Guangxi, People's Republic of China
通讯作者：
通讯机构：[4]Department of Pharmacy, The General Hospital of the Chinese People's Armed Police Forces, Beijing, People's Republic of China[5]Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China[*1]Department of Pharmacy, The General Hospital of the Chinese People's Armed Police Forces, Yongding road, No. 69, Haidian District, Beijing, 100039 People's Republic of China[*2]Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Tiantan Xili No. 6, Dongcheng District, Beijing, 100050 People's Republic of China
推荐引用方式(GB/T 7714)：
Mao Guifu,Zhao Kun,Sun Shusen,et al.A new derivatization method for the determination of valproic acid in serum using tetramethylammonium hydroxide as a catalyst[J].BIOMEDICAL CHROMATOGRAPHY.2019,33(3):doi:10.1002/bmc.4440.
APA：
Mao, Guifu,Zhao, Kun,Sun, Shusen,Lu, Yanxia&Li, Xingang.(2019).A new derivatization method for the determination of valproic acid in serum using tetramethylammonium hydroxide as a catalyst.BIOMEDICAL CHROMATOGRAPHY,33,(3)
MLA：
Mao, Guifu,et al."A new derivatization method for the determination of valproic acid in serum using tetramethylammonium hydroxide as a catalyst".BIOMEDICAL CHROMATOGRAPHY 33..3(2019)
