单位：[1]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical ResearchCenter for Digestive Diseases, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, Beijing 100050临床科室国家中心消化分中心消化内科首都医科大学附属北京友谊医院[2]Department of Digestive Diseases,Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China[3]Molecular Medicine Experimental Center,Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
In recent years, microRNA-193b (miR-193b) is regarded as a tumor suppressor in the development and progression of various cancers. Several studies have indicated that KRAS could be regulated by miR-193b in pancreatic cancer cells. However, the function of miR-193b in human esophageal squamous cell carcinoma has not been explored intensively thus far. Herein, the relationship between miR-193b and KRAS was mainly explored in esophageal squamous cell carcinoma cells. In the present study, the expression levels of miR-193b and KRAS were assessed in both human esophageal cancer cells and tissues. The direct regulatory relationship between miR-193b and KRAS was evaluated using dual-luciferase assay. The effect of miR-193b overexpression and inhibitor on cell proliferation, migration/invasion, and apoptosis was further detected herein. Our results indicated that the expression of miR-193b was significantly lower in human esophageal cancer tissues than paracancerous tissues. The expression level of miR-193b/KRAS was stage-dependent in human esophageal cancers. KRAS was indicated as the direct target of miR-193b, and upregulation of miR-193b increased the percentage of cell apoptosis, and suppressed cell proliferation as well as cell migration/invasion via direct regulation of KRAS. Therefore, our study indicated that miR-193b plays an important role in the development and progression of human esophageal squamous cell carcinoma, which may become a novel target in the treatment of human esophageal squamous cell carcinoma in the future.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570507, 81670474, 81302160]
第一作者单位：[1]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical ResearchCenter for Digestive Diseases, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, Beijing 100050[2]Department of Digestive Diseases,Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
通讯作者：
通讯机构：[1]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical ResearchCenter for Digestive Diseases, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, Beijing 100050[*1]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Diseases, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, 95 Yong'an Road, Xicheng, Beijing 100050, P.R. China
推荐引用方式(GB/T 7714)：
MIN KANG,YAN LI,SHENGTAO ZHU,et al.MicroRNA-193b acts as a tumor suppressor gene in human esophageal squamous cell carcinoma via target regulation of KRAS[J].ONCOLOGY LETTERS.2019,17(4):3965-3973.doi:10.3892/ol.2019.10039.
APA：
MIN KANG,YAN LI,SHENGTAO ZHU,SHUTIAN ZHANG,SHUILONG GUO&PENG LI.(2019).MicroRNA-193b acts as a tumor suppressor gene in human esophageal squamous cell carcinoma via target regulation of KRAS.ONCOLOGY LETTERS,17,(4)
MLA：
MIN KANG,et al."MicroRNA-193b acts as a tumor suppressor gene in human esophageal squamous cell carcinoma via target regulation of KRAS".ONCOLOGY LETTERS 17..4(2019):3965-3973
