Molecular characterization and analysis of Mycoplasma pneumoniae among patients of all ages with community-acquired pneumonia during an epidemic in China
单位：[1]Department of Clinical Laboratory, The Third People’s Hospital of Shenzhen, Shenzhen, Guangdong, China深圳市康宁医院深圳医学信息中心[2]Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital MedicalUniversity, Beijing, China北京朝阳医院[3]Department of Clinical Trial, The Third People’s Hospital of Shenzhen, Shenzhen, Guangdong, China深圳市康宁医院深圳医学信息中心[4]Department of Pediatrics, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital Medical University, Beijing, China首都儿科研究所北京朝阳医院[5]Department of Respiratory and Critical Care Medicine, Clinical Microbiology and Infectious Disease Lab, China-Japan Friendship Hospital, Beijing, China
Background: Analysis of the molecular characteristics of isolates is very important for clinical and epidemiological study of community-acquired pneumonia (CAP) caused by Mycoplasma pneumoniae. Methods: Between 2010 and 2012, an epidemic period, M. pneumoniae was isolated from oropharyngeal swabs of consecutive CAP patients. Minimum inhibitory concentrations of macrolides, 23S rRNA gene sequencing, P1 gene and multilocus variable-number tandem-repeat analysis (MLVA) genotyping was conducted. Results: 88.3% (181/205) of the isolates were macrolide-resistant M. pneumoniae (MRMP) and all harbored an A2063 G mutation. The strains were clustered into 7 MLVA types, and P1 type 1 and type 2 lineages were co-circulated (86.3% and 13.7%). Compared with adults, no specific MLVA type contributed to higher M. pneumoniae infection in children (p = 0.14). Similar macrolide profile and genotypes of M. pneumoniae was found between outpatients and inpatients. Significant differences in proportion of P1 types and two main MLVA types 4/5/7/2 and 3/5/6/2 were observed between MRMP and macrolide-sensitive M. pneumoniae (MSMP) (p < 0.001). Conclusions: This study demonstrates a comprehensive profile of M. pneumoniae molecular characterization among CAP patients of all age, and provides more evidences on a correlation between MLVA type 4/5/7/2 and macrolide resistance in the setting of high incidence of MRMP. (c) 2019 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81400005/H0104]; Beijing Municipal Administration of Hospitals' Youth Programme [20150306]; Shenzhen Key Laboratory of Biochip [ZDSYS201504301534057]; Special Support Fund of Shenzhen for Introduced High-Level Medical Team, Translational Medicine of Biochip in Clinical Laboratory [SZSM201412005]
第一作者单位：[1]Department of Clinical Laboratory, The Third People’s Hospital of Shenzhen, Shenzhen, Guangdong, China[2]Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital MedicalUniversity, Beijing, China[*1]Department of Clinical Laboratory, The Third People’s Hospital of Shenzhen, Shenzhen, Guangdong, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, The Third People’s Hospital of Shenzhen, Shenzhen, Guangdong, China[2]Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital MedicalUniversity, Beijing, China[*1]Department of Clinical Laboratory, The Third People’s Hospital of Shenzhen, Shenzhen, Guangdong, China
推荐引用方式(GB/T 7714)：
Qu Jiuxin,Chen Shuyan,Bao Fang,et al.Molecular characterization and analysis of Mycoplasma pneumoniae among patients of all ages with community-acquired pneumonia during an epidemic in China[J].INTERNATIONAL JOURNAL of INFECTIOUS DISEASES.2019,83:26-31.doi:10.1016/j.ijid.2019.03.028.
APA：
Qu, Jiuxin,Chen, Shuyan,Bao, Fang,Gu, Li&Cao, Bin.(2019).Molecular characterization and analysis of Mycoplasma pneumoniae among patients of all ages with community-acquired pneumonia during an epidemic in China.INTERNATIONAL JOURNAL of INFECTIOUS DISEASES,83,
MLA：
Qu, Jiuxin,et al."Molecular characterization and analysis of Mycoplasma pneumoniae among patients of all ages with community-acquired pneumonia during an epidemic in China".INTERNATIONAL JOURNAL of INFECTIOUS DISEASES 83.(2019):26-31
