单位：[1]Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Department of Gastroenterology, Beijing Friendship Hospital, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, Capital MedicalUniversity, Beijing, China临床科室国家中心消化分中心消化内科首都医科大学附属北京友谊医院[2]Department of Operations and Information Management, China-Japan Friendship Hospital,Beijing, China[3]MOE Key Laboratory of Bioinformatics, BNRist, Department of Automation, Bioinformatics Division, Centerfor Synthetic and Systems Biology, Tsinghua University, Beijing, China
Irritable bowel syndrome (IBS) is a common gastrointestinal dysfunctional disease. The pathophysiology of IBS is, however, largely unknown. This study aimed to determine whether evaluation of fecal metabolite and microbiota profiles may offer an opportunity to identify a novel pathophysiological target for IBS, and to reveal possible gut microbe-metabolite associations. By using gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and 16S rRNA gene sequencing, we measured fecal metabolites and microbiota of the control and water avoidance stress (WAS)-induced IBS rats. We found a significantly differential metabolite profile between the IBS and control groups; a cluster of metabolites was also found to be significantly associated with the amount of defecations. Moreover, the WAS group exhibited a decreased alpha diversity of the microbial population as compared to the control group. However, the characteristics of gut microbiota could not differentiate the IBS group from the control group. Correlation of the metabolite level with the number of microbial genera showed no significant association between the control and IBS groups. This study provides a global perspective on metabolomics and microbiota profiling in WAS-induced IBS model and a theoretical basis for research on the pathophysiology of IBS.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81802310]; Beijing Municipal Administration of Hospitals' Youth Program [QML20180108]; The Digestive Medical Coordinated Development Center of Beijing Municipal Administration of Hospitals [XXZ01]
第一作者单位：[1]Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Department of Gastroenterology, Beijing Friendship Hospital, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, Capital MedicalUniversity, Beijing, China
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推荐引用方式(GB/T 7714)：
Liu Si,Si Chaozeng,Yu Yang,et al.Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome[J].FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY.2019,9:doi:10.3389/fcimb.2019.00178.
APA：
Liu, Si,Si, Chaozeng,Yu, Yang,Zhao, Guiping,Chen, Lei...&Zhu, Shengtao.(2019).Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome.FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY,9,
MLA：
Liu, Si,et al."Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome".FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY 9.(2019)
