单位：[a]Department of Cardiology, Emergency General Hospital, Beijing 100028, China[b]Department of Geriatric, Peking University First Hospital, Beijing 100034, China[c]Department of Research Ward, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China临床科室研究型病房研究型病房首都医科大学附属北京友谊医院[d]Department of Cardiology, Fenyang Hospital of Shanxi Province, Fenyang 032200, China
We examined the effect of ginsenoside Re (G-Re) on autophagy in H9c2 cardiomyocytes cultured in glucose deprivation (GD). Levels of the membrane-bound autophagy-related microtubule-associated protein 1A/1B-light chain 3 (LC3) B-2 were measured via immunoblotting and immunofluorescence was conducted to assess autophagosome formation. GD H9c2 cells were treated with 100 mu mol/l G-Re. Cell viability was determined in culture medium. Phosphorylated 5 ' AMP-activated protein kinase (AMPK)-alpha and mammalian target of rapamycin (mTOR) levels were measured to explore the mechanisms underlying the effects of G-Re on autophagy in GD cells. G-Re treatment inhibited autophagosome formation and may be beneficial to GD cardiomyocytes.
基金：
National Science and Technology Major Projects for "Major New Drugs Innovation and Development" [2009ZX09103-441]
第一作者单位：[a]Department of Cardiology, Emergency General Hospital, Beijing 100028, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zi-Long Zhang,Mei-Lin Liu,Ying-Shuo Huang,et al.Ginsenoside Re enhances the survival of H9c2 cardiac muscle cells through regulation of autophagy[J].JOURNAL of ASIAN NATURAL PRODUCTS RESEARCH.2020,22(8):774-787.doi:10.1080/10286020.2019.1632834.
APA：
Zi-Long Zhang,Mei-Lin Liu,Ying-Shuo Huang,Wen-Yi Liang,Miao-Miao Zhang...&Ming-Feng Ma.(2020).Ginsenoside Re enhances the survival of H9c2 cardiac muscle cells through regulation of autophagy.JOURNAL of ASIAN NATURAL PRODUCTS RESEARCH,22,(8)
MLA：
Zi-Long Zhang,et al."Ginsenoside Re enhances the survival of H9c2 cardiac muscle cells through regulation of autophagy".JOURNAL of ASIAN NATURAL PRODUCTS RESEARCH 22..8(2020):774-787
