单位：[1]General Surgery Department, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China临床科室国家中心普外分中心普外五科（综合普外科）首都医科大学附属北京友谊医院[2]Experimental and Translational Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China医技科室北京市临床医学研究所实验中心首都医科大学附属北京友谊医院[3]Beijing Clinical Research Institute, Beijing 100050, China[4]Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing 100050, China[5]National Clinical Research Center for Digestive Diseases, Beijing 100050, China.首都医科大学附属北京友谊医院
Allergic asthma is an inflammatory disorder of the airway without satisfactory traditional therapies capable of controlling the underlying pathology. New approaches that can overcome the detrimental effects of immune dysregulation are thus desirable. Here we adoptively transfer ovalbumin (OVA) peptide-primed CD4(-) CD8(-) double negative T (DNT) cells intravenously into a mouse model of OVA-induced allergic asthma to find that OVA-induced airway hyperresponsiveness, lung inflammation, mucus production and OVA-specific IgG/IgE production are significantly suppressed. The immunosuppressive function of the OVA-specific DNT cells is dependent on the inhibition of CD11b+ dendritic cell function, T follicular helper cell proliferation, and IL-21 production. Mechanistically, Lag3 contributes to MHC-II antigen recognition and trogocytosis, thereby modulating the antigen-specific immune regulation by DNT cells. The effectiveness of ex vivo-generated allergen-specific DNT cells in alleviating airway inflammation thus supports the potential utilization of DNT cell-based therapy for the treatment of allergic asthma.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81601388, 81870399]
第一作者单位：[1]General Surgery Department, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China[2]Experimental and Translational Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China[3]Beijing Clinical Research Institute, Beijing 100050, China[4]Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing 100050, China
通讯作者：
通讯机构：[1]General Surgery Department, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China[2]Experimental and Translational Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China[3]Beijing Clinical Research Institute, Beijing 100050, China[4]Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing 100050, China[5]National Clinical Research Center for Digestive Diseases, Beijing 100050, China.
推荐引用方式(GB/T 7714)：
Tian Dan,Yang Lu,Wang Song,et al.Double negative T cells mediate Lag3-dependent antigen-specific protection in allergic asthma[J].NATURE COMMUNICATIONS.2019,10:doi:10.1038/s41467-019-12243-0.
APA：
Tian, Dan,Yang, Lu,Wang, Song,Zhu, Yanbing,Shi, Wen...&Zhang, Dong.(2019).Double negative T cells mediate Lag3-dependent antigen-specific protection in allergic asthma.NATURE COMMUNICATIONS,10,
MLA：
Tian, Dan,et al."Double negative T cells mediate Lag3-dependent antigen-specific protection in allergic asthma".NATURE COMMUNICATIONS 10.(2019)
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