单位：[1]Department of Pulmonary and Critical Care Medicine.Center of Respiratory Medicine, China-Japan Friendship Hospital.National Clinical Research Center for Respiratory Diseases.Institute of Respiratory Medicine Chinese Academy of Medical Sciences, Beijing 100029, China[2]Graduate School of Peking Union Medical College, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100005, China[3]National Institute of Biological Sciences, Beijing 102206, China
The aging of alveolar stem cells has been linked to many chronic lung diseases, including pulmonary fibrosis. However, the effects of aging on alveolar stem cells during homeostasis and post-injury alveolar repair have not been well characterized. Here we conducted a single-cell RNA sequencing (scRNA-seq) analysis of alveolar stem cells of 3-month-old and 12-month-old mice to characterize the aging effect on alveolar stem cells. Our results have shown that the transcriptomes of alveolar stem cells of 3-month-old and 12-month-old mice are not significantly different under the steady condition. However, after a bleomycin-induced lung injury, the alveolar stem cells of 12-month-old mice show enhanced inflammatory responses and decreased lipid metabolism. Our study suggests a close relationship among aging, lipid metabolism, inflammatory responses and chronic lung diseases.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81430001, 81870056]; Beijing Major Science and Technology Projects [Z171100000417003]
第一作者单位：[1]Department of Pulmonary and Critical Care Medicine.Center of Respiratory Medicine, China-Japan Friendship Hospital.National Clinical Research Center for Respiratory Diseases.Institute of Respiratory Medicine Chinese Academy of Medical Sciences, Beijing 100029, China[2]Graduate School of Peking Union Medical College, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100005, China
通讯作者：
通讯机构：[1]Department of Pulmonary and Critical Care Medicine.Center of Respiratory Medicine, China-Japan Friendship Hospital.National Clinical Research Center for Respiratory Diseases.Institute of Respiratory Medicine Chinese Academy of Medical Sciences, Beijing 100029, China[2]Graduate School of Peking Union Medical College, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100005, China
推荐引用方式(GB/T 7714)：
Lv Tingting,Jiang Kewu,Wang Jiawen,et al.Single-cell RNA sequencing profiling of the effects of aging on alveolar stem cells[J].SCIENCE CHINA-LIFE SCIENCES.2019,62(8):1028-1037.doi:10.1007/s11427-019-9583-9.
APA：
Lv, Tingting,Jiang, Kewu,Wang, Jiawen,Tang, Nan,Dai, Huaping&Wang, Chen.(2019).Single-cell RNA sequencing profiling of the effects of aging on alveolar stem cells.SCIENCE CHINA-LIFE SCIENCES,62,(8)
MLA：
Lv, Tingting,et al."Single-cell RNA sequencing profiling of the effects of aging on alveolar stem cells".SCIENCE CHINA-LIFE SCIENCES 62..8(2019):1028-1037
生物学