Overexpression of FER1L4 promotes the apoptosis and suppresses epithelial-mesenchymal transition and stemness markers via activating PI3K/AKT signaling pathway in osteosarcoma cells
单位：[1]Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical University, 100050, PR China临床科室骨科骨科首都医科大学附属北京友谊医院[2]Department of Medicine, College of Medicine and Life Sciences, University of Toledo, Ohio, 43614, USA
Novel long non-coding RNA Fer-l-like protein 4 (FER1L4) has been identified as a tumor suppressor in endometrial carcinoma, ovarian cancer, hepatocellular carcinoma, esophageal squamous cell carcinoma. However, the function of FER1L4 in osteosarcoma has not been clear. The aim of the research was to explore the effects of FER1L4 in osteosarcoma. Results showed that FER1L4 was observed to be lowly expressed in osteosarcoma cell lines (US-O2, MG-63 and SaOS-2 cells), especially MG63 cells. Besides, overexpression of FER1L4 remarkably repressed the proliferation, migration and invasion of MG63 cells. FER1L4-induced apoptotic cell death leaded to the activation of caspase-3 and Bax/Bcl2. Moreover, epithelial-mesenchymal transition (EMT) was tremendously suppressed by increased FER1L4, evidences were the increased E-cadherin and reduced vimentin and fibronectin. Blocking FER1L4 expression by sh-FER1L4 treatment increased the expression of SOX9, CD44, ALDH1, Nanog and Oct4, indicating that FER1L4 could effectively decrease cell stemness in osteosarcoma. Furthermore, the protein levels of p-AKT and p-PI3K were remarkably suppressed when FER1L4 was knocked down. In conclusion, the study indicated that FER1L4 acted as a tumor suppressor in osteosarcoma via activating PI3K/AKT pathway may be a new prognostic biomarker and potential therapeutic target for osteosarcoma intervention.
第一作者单位：[1]Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical University, 100050, PR China
通讯作者：
通讯机构：[1]Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical University, 100050, PR China[*1]No. 95 Yong An Road, Xi Cheng District, Beijing, 100050, PR China.
推荐引用方式(GB/T 7714)：
Ma Lifeng,Zhang Liang,Guo Ai,et al.Overexpression of FER1L4 promotes the apoptosis and suppresses epithelial-mesenchymal transition and stemness markers via activating PI3K/AKT signaling pathway in osteosarcoma cells[J].PATHOLOGY RESEARCH and PRACTICE.2019,215(6):doi:10.1016/j.prp.2019.04.004.
APA：
Ma, Lifeng,Zhang, Liang,Guo, Ai,Liu, Lijun C.,Yu, Fei...&Wang, Difan.(2019).Overexpression of FER1L4 promotes the apoptosis and suppresses epithelial-mesenchymal transition and stemness markers via activating PI3K/AKT signaling pathway in osteosarcoma cells.PATHOLOGY RESEARCH and PRACTICE,215,(6)
MLA：
Ma, Lifeng,et al."Overexpression of FER1L4 promotes the apoptosis and suppresses epithelial-mesenchymal transition and stemness markers via activating PI3K/AKT signaling pathway in osteosarcoma cells".PATHOLOGY RESEARCH and PRACTICE 215..6(2019)
