单位：[1]State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China[2]Research Institute of Shenzhen Jinxinnong Technology CO., LTD., Shenzhen 518106, China[3]Department of Obstetrics and Gynecology, China-Japan Friendship Hospital, Beijing 100029, China[4]Department of Animal Science, Texas A&M University, College Station, TX 77843, USA
Maternal dietary supplementation with l-glutamine (Gln) has been considered as an option to improve fetal growth and to prevent the occurrence of intrauterine growth restriction (IUGR). This study investigated whether maternal Gln supplementation could improve fetal growth as well as the intestinal development during late pregnancy. Sixty pregnant Landrace x Large White multiparous sows were assigned to two groups, either the group fed the control diet or the group with the diet supplemented with 1% Gln from d 85 of gestation until farrowing. One normal body weight piglet and one IUGR piglet were obtained from six litters in each group. Reproductive performance, plasma concentrations of free amino acids and related metabolites as well as piglet growth and tissue indexes were determined. Maternal Gln supplementation during late gestation increased the average birth weight, while decreasing the within-litter variation of newborn piglets. The concentrations of Gln in plasma were lower in IUGR piglets than in normal piglets. Glutamine supplementation enhanced Gln concentrations in maternal and piglet plasma and the piglet jejunum, compared with the Control group. Supplementing Gln suppressed intestinal miR-29a levels, and increased the abundance of extracellular matrix (ECM) and tight junction (TJ) proteins, resulting in increased intestinal weight and improved morphologies of the piglets. Collectively, Gln supplementation to the sow's diet increased fetal growth, decreased the within-litter variation of newborn piglets, and alleviated the IUGR-induced intestinal impairment. These findings suggest the possibility of maternal glutamine supplementation in the prevention and treatment of IUGR in animal production and human medicine.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31272449, 31422052, 31630074]; Beijing Municipal Natural Science FoundationBeijing Natural Science Foundation [S170001]; National Key Research and Development Program of China [2016YFD0500506]; 111 ProjectMinistry of Education, China - 111 Project [B16044]; Jinxinnong Animal Science Developmental Foundation; Hunan Co-Innovation Center of Animal Production Safety, CICAPS
第一作者单位：[1]State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China[2]Research Institute of Shenzhen Jinxinnong Technology CO., LTD., Shenzhen 518106, China
通讯作者：
推荐引用方式(GB/T 7714)：
Yuhua Zhu,Tiantian Li,Shimeng Huang,et al.Maternal l-glutamine supplementation during late gestation alleviates intrauterine growth restriction-induced intestinal dysfunction in piglets[J].AMINO ACIDS.2018,50(9):1289-1299.doi:10.1007/s00726-018-2608-5.
APA：
Yuhua Zhu,Tiantian Li,Shimeng Huang,Wei Wang,Zhaolai Dai...&Junjun Wang.(2018).Maternal l-glutamine supplementation during late gestation alleviates intrauterine growth restriction-induced intestinal dysfunction in piglets.AMINO ACIDS,50,(9)
MLA：
Yuhua Zhu,et al."Maternal l-glutamine supplementation during late gestation alleviates intrauterine growth restriction-induced intestinal dysfunction in piglets".AMINO ACIDS 50..9(2018):1289-1299
