单位：[1]Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China[2]Department of Gastroenterology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China[3]Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China[4]Basic Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China[5]Traditional Chinese Medicine Department of Gastroenterology, China-Japan Friendship Hospital, Beijing 100029, China[6]College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China[7]Division of Gastroenterology and Hepatology, Johns Hopkins University, School of Medicine, Baltimore, MD 21224, USA
Objective. The aim of this study was to examine a new method to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome. Methods. A senna leaf (Folium sennae) decoction was made in 3 concentrations of 1.0, 0.5, and 0.25 g/mL. Rats were randomly divided into 4 groups: the control (C)-, high (H)-, middle (M)-, and low (L)-dose groups. The groups received saline, 1.0, 0.5, or 0.25 g/mL senna leaf decoction, respectively, for 4 weeks. Body weight monitoring, food consumption, water intake, defecation frequency, stool Bristol score, weight-loaded forced swimming test, forelimb grip strength test, D-xylose absorption test, serum cortisone, adrenocorticotropic hormone (ACTH), 24 h urine 17-hydroxycorticosteroid (17-OHCS), and histopathological detection were conducted to assess the success of the senna leaf decoction-induced model. Results. This study showed that the senna leaf decoction could induce diarrhea and dose-dependently slow body weight growth, reduce food consumption, and increase water intake, stool Bristol score, and defecation frequency. Statistical differences were found between groups H and M in rectal temperature, weight-loaded forced swimming time, forelimb grip strength, and serum cortisone. The D-xylose absorption test also showed dysfunction of intestinal absorption in groups H and M. The serum cortisone and 24 h urine 17-OHCS were significantly reduced in group H. Conclusions. Gastric gavage of 10 mL/kg of body weight of a high concentration of a senna leaf decoction (1.0 g/mL) for 4 weeks was used to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81774066, 81703923, 81573806, 81403389]; Foundation on Beijing Key Laboratory of Functional Gastrointestinal Disorders Diagnosis and Treatment of Traditional Chinese Medicine [BZ0374]
第一作者单位：[1]Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China[2]Department of Gastroenterology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China[3]Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhu Jiajie,Liu Shan,Guo Yu,et al.A New Model of Diarrhea with Spleen-Kidney Yang Deficiency Syndrome[J].EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE.2018,2018:doi:10.1155/2018/4280343.
APA：
Zhu, Jiajie,Liu, Shan,Guo, Yu,Hou, Liwei,Su, Xiaolan...&Wei, Wei.(2018).A New Model of Diarrhea with Spleen-Kidney Yang Deficiency Syndrome.EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE,2018,
MLA：
Zhu, Jiajie,et al."A New Model of Diarrhea with Spleen-Kidney Yang Deficiency Syndrome".EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE 2018.(2018)
