单位：[1]International Medical Department, China-Japan Friendship Hospital, Beijing, China[2]School of Basic Medicine, Beijing University of Chinese Medicine, Beijing, China[3]Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, Jiaxing, China[4]Department of Gastroenterology, China-Japan Friendship Hospital, Beijing, China
Baicalin, a flavonoid isolated from Scutellaria baicalensis Georgi, has shown a wide range of anti-inflammatory, antioxidative, antiviral, and antitumor properties. However, the molecular mechanism of how baicalin exerts its effects, especially on inflammation regulation, has not been fully investigated. In this article, we report the effects of baicalin on the mouse macrophage cell line RAW264.7. Our results demonstrate that baicalin inhibits the production of inflammatory factors interleukin-6 and tumor necrosis factor-alpha upon lipopolysaccharide stimulation of macrophages. We observed that baicalin inhibits STAT3 activation through retarding its expression and phosphorylation. Interestingly, baicalin treatment promotes the elevation of miR-124 in lipopolysaccharide-treated macrophages. Overexpression of the miR-124 mimic in RAW264.7 reduced STAT3 expression and phosphorylation. Furthermore, inhibition of miR-124 attenuated the dysregulation of STAT3 and reduction of inflammatory factors upon baicalin treatment. Our results revealed the molecular mechanism that baicalin attenuates pro-inflammatory cytokine production through miR-124-STAT3 signaling pathway, suggesting that miR-124 is an important modulator in regulating anti-inflammation by baicalin in macrophages.
基金：
Research Fund of China-Japan Friendship Hospital [2017-2-QN-18]
第一作者单位：[1]International Medical Department, China-Japan Friendship Hospital, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[3]Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, Jiaxing, China[4]Department of Gastroenterology, China-Japan Friendship Hospital, Beijing, China[*1]Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 705 Yatai Road, Jiaxing 314006, Zhejiang, China[*2]Department of Gastroenterology, China-Japan Friendship Hospital, No. 2 Yinghua East Road, Beijing 100029, China
推荐引用方式(GB/T 7714)：
Tang Bin,Wang Xixi,Zhu Yuqing,et al.Baicalin attenuates lipopolysaccharide-induced pro-inflammatory cytokine expression in murine macrophage cell line RAW264.7 through miR-124-STAT3 axis[J].EUROPEAN JOURNAL of INFLAMMATION.2018,16:doi:10.1177/2058739218798463.
APA：
Tang, Bin,Wang, Xixi,Zhu, Yuqing,Li, Xuhui&Yao, Shukun.(2018).Baicalin attenuates lipopolysaccharide-induced pro-inflammatory cytokine expression in murine macrophage cell line RAW264.7 through miR-124-STAT3 axis.EUROPEAN JOURNAL of INFLAMMATION,16,
MLA：
Tang, Bin,et al."Baicalin attenuates lipopolysaccharide-induced pro-inflammatory cytokine expression in murine macrophage cell line RAW264.7 through miR-124-STAT3 axis".EUROPEAN JOURNAL of INFLAMMATION 16.(2018)
