Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway
单位：[1]College of Life Science and Technology, Dalian University, Liaoning, 116622, China[2]Department of Engineering Physics, Institute of Medical Physics, Tsinghua University, Beijing, 100084, China[3]Division of Medical Oncology, Department of Integrated Traditional and Western Medicine, China-Japan Friendship Hospital, Beijing, 100029, China
The inflammatory cellular model of RAW264.7 cells induced by lipopolysaccharide (LPS) has always been used to investigate the effect of anti-inflammatory agents in vitro. In the present study, the anti-inflammatory activity of total flavones extracted from the fermentation broth of the co-culture of Coprinus comatus and Morchella esculenta (MCF-F), and its potential molecular mechanism in LPS-challenged RAW264.7 macrophage cells were investigated. The data revealed that MCF-F exhibited anti-inflammatory activity in LPS-stimulated RAW264.7 cells. At the same time, MCF-F was less cytotoxic under a concentration of 16 mu g/ml in RAW264.7 cells. The anti-inflammatory activity of MCF-F was detected using the Griess method and ELISA assay, and the results well-corroborated with the observed decrease in expression in pro-inflammatory mediators, including nitric oxide, tumor necrosis factor-alpha and inteleukin-1 beta (IL-1 beta). In addition, the expression of inducible NO synthase (iNOS) and cyclooxygenase2 (COX-2) were confirmed by RT-PCR and western blot, and it was found that both mRNA and protein levels were downregulated after MCF-F treatment. The data also revealed that MCF-F downregulated the phosphorylation of JNK, ERK and P38 MAPK. Collectively, these results lead to the conclusion that MCF-F exerts an anti-inflammatory effect against LPS-challenged RAW264.7 cells via the MAPK pathway.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21402104, 81570188]
第一作者单位：[1]College of Life Science and Technology, Dalian University, Liaoning, 116622, China
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推荐引用方式(GB/T 7714)：
Zhao Xiaohong,Zou Xianwei,Li Qian,et al.Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway[J].MICROBIAL PATHOGENESIS.2018,125:431-437.doi:10.1016/j.micpath.2018.10.008.
APA：
Zhao, Xiaohong,Zou, Xianwei,Li, Qian,Cai, Xu,Li, Liya...&Wang, Huiguo.(2018).Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.MICROBIAL PATHOGENESIS,125,
MLA：
Zhao, Xiaohong,et al."Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway".MICROBIAL PATHOGENESIS 125.(2018):431-437
