单位：[1]Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University,Beijing 100191, China[2]Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing100191, China[3]Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of thePeople’s Republic of China, Beijing 100191, China[4]Key Laboratory of Stasis and Phlegm, State Administrationof Traditional Chinese Medicine of the People’s Republic of China, Beijing 100191, China[5]Beijing Laboratory ofIntegrative Microangiopathy, Beijing 100191, China[6]Department of Cardiology, Beijing China-Japan FriendshipHospital, Beijing 100029, China
Cardiac ischemia and reperfusion (I/R) injury remains a challenge for clinicians. Ginsenoside Rb1 (Rb1) has been reported to have the ability to attenuate I/R injury, but its effect on energy metabolism during cardiac I/R and the underlying mechanism remain unknown. In this study, we detected the effect of Rb1 on rat myocardial blood flow, myocardial infarct size, cardiac function, velocity of venule red blood cell, myocardial structure and apoptosis, energy metabolism and change in RhoA signaling pathway during cardiac I/R injury. In addition, the binding affinity of RhoA to Rb1 was detected using surface plasmon resonance (SPR). Results showed that Rb1 treatment at 5 mg/kg/h protected all the cardiac injuries induced by I/R, including damaged myocardial structure, decrease in myocardial blood flow, impaired heart function and microcirculation, cardiomyocyte apoptosis, myocardial infarction and release of myocardial cTnI. Rb1 also inhibited the activation of RhoA signaling pathway and restored the production of ATP during cardiac I/R. Moreover, SPR assay showed that Rb1 was able to bind to RhoA in a dose-dependent manner. These results indicate that Rb1 may prevent I/R-induced cardiac injury by regulation of RhoA signaling pathway, and may serve as a potential regime to improve percutaneous coronary intervention outcome.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81273637]; Production of New Medicine Program of Ministry of Science and Technology of China [2013ZX09402202]
第一作者单位：[1]Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University,Beijing 100191, China[2]Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing100191, China[3]Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of thePeople’s Republic of China, Beijing 100191, China[4]Key Laboratory of Stasis and Phlegm, State Administrationof Traditional Chinese Medicine of the People’s Republic of China, Beijing 100191, China[5]Beijing Laboratory ofIntegrative Microangiopathy, Beijing 100191, China
通讯作者：
通讯机构：[1]Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University,Beijing 100191, China[2]Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing100191, China[3]Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of thePeople’s Republic of China, Beijing 100191, China[4]Key Laboratory of Stasis and Phlegm, State Administrationof Traditional Chinese Medicine of the People’s Republic of China, Beijing 100191, China[5]Beijing Laboratory ofIntegrative Microangiopathy, Beijing 100191, China
推荐引用方式(GB/T 7714)：
Cui Yuan-Chen,Pan Chun-Shui,Yan Li,et al.Ginsenoside Rb1 protects against ischemia/reperfusion-induced myocardial injury via energy metabolism regulation mediated by RhoA signaling pathway[J].SCIENTIFIC REPORTS.2017,7:doi:10.1038/srep44579.
APA：
Cui, Yuan-Chen,Pan, Chun-Shui,Yan, Li,Li, Lin,Hu, Bai-He...&Han, Jing-Yan.(2017).Ginsenoside Rb1 protects against ischemia/reperfusion-induced myocardial injury via energy metabolism regulation mediated by RhoA signaling pathway.SCIENTIFIC REPORTS,7,
MLA：
Cui, Yuan-Chen,et al."Ginsenoside Rb1 protects against ischemia/reperfusion-induced myocardial injury via energy metabolism regulation mediated by RhoA signaling pathway".SCIENTIFIC REPORTS 7.(2017)
