单位：[1]Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China临床科室神经内科神经内科首都医科大学附属北京友谊医院[2]Atlanta, GA, USA[3]Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China临床科室国家中心肝病分中心首都医科大学附属北京友谊医院[4]Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China医技科室药学部首都医科大学附属北京友谊医院[5]China Institute of Sports Medicine, 2-A Sidegate, Tiyuguan Road, Dongcheng District, Beijing 100061, China
This study investigated the mechanisms underlying regulation of the serotonin system in the rat brain during exercise-induced chronic fatigue. High-performance liquid chromatography-mass spectrometry (HPLC-MS) was performed to measure serum tryptophan of the fatigued rat. HPLC was conducted to measure 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in the frontal cortex and hippocampus. In addition, 5-HT1A receptor and 5-HT transporter (5-HTT) mRNA expressions were measured at the same locations using real-time PCR. The results demonstrated a significant reduction in the serum tryptophan level in rats with exercise-induced chronic fatigue. Moreover, increased 5-HT and decreased 5-HIAA levels were detected in the frontal cortex and hippocampus, and these alterations were significant. Further, 5-HTT expression was significantly increased and 5-HT1A receptor expression was significantly decreased. These results indicate that the 5-HT system plays an important role in the development of exercise-induced chronic fatigue. The 5-HT levels in different parts of the brain increased simultaneously, especially at synapses, and these alterations were associated with changes in 5-HTT and 5-HT1A mRNA expressions. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.
基金：
Specialized Research Fund of the National Natural Science Foundation of China [SN 81041019]; National Science and Technology Major Project [SN 2012BAK21B02]
第一作者单位：[1]Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
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推荐引用方式(GB/T 7714)：
Liu Zhandong,Wu Yanjue,Liu Tianhui,et al.SEROTONIN REGULATION IN A RAT MODEL OF EXERCISE-INDUCED CHRONIC FATIGUE[J].NEUROSCIENCE.2017,349:27-34.doi:10.1016/j.neuroscience.2017.02.037.
APA：
Liu, Zhandong,Wu, Yanjue,Liu, Tianhui,Li, Ren&Xie, Minhao.(2017).SEROTONIN REGULATION IN A RAT MODEL OF EXERCISE-INDUCED CHRONIC FATIGUE.NEUROSCIENCE,349,
MLA：
Liu, Zhandong,et al."SEROTONIN REGULATION IN A RAT MODEL OF EXERCISE-INDUCED CHRONIC FATIGUE".NEUROSCIENCE 349.(2017):27-34
医学