单位：[1]Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China [2]Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China [3]China National Health Development Research Center, Beijing 100019, China [4]China-Japan Friendship Hospital, Beijing 100029, China
Erchen decoction (ECD) and Linguizhugan decoction (LGZGD), both are Chinese herbal formula, have been used clinically for the treatment of nonalcoholic fatty liver disease (NAFLD). However, their therapeutic mechanisms are still unclear. Because insulin resistance (IR) is a key etiological factor in the pathology of high-fat diet- (HFD-) induced NAFLD, in this study, the protective effects of ECD and LGZGD on HFD-induced insulin resistance in rats were evaluated and their mechanisms were investigated by OGTT and Western blot. The results showed that treatment with ECD and LGZGD significantly improved insulin resistance and liver damage in rats, evidenced by supported serum aminotransferase levels and the histopathological examination. ECD and LGZGD also showed significant protective effects against HFD-induced hyperlipidemia and the inhibition of the hepatocyte proliferation by palmitate. Furthermore, supplementation of ECD and LGZGD decreased TNF-alpha, NF-kappa B, and IRS-1Ser307 phosphorylation expressions in vivo and in vitro. These results indicated that ECD and LGZGD have protective effects against HFD-induced liver IR and their underlying mechanisms involve the TNF-alpha and insulin pathway. These findings would be beneficial for understanding of the therapeutic effects of ECD and LGZGD in treatment of NAFLD.
基金：
Beijing Municipal Administration of Hospitals [2016013]; Cooperation Fund of Basic and Clinical Research of Capital Medical University [16JL24]; Beijing Hospital of Traditional Chinese Medicine [YJ-201722]; Fund for Beijing Science & Technology Development of TCM [QN2016-24]
第一作者单位：[1]Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China [2]Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China
通讯作者：
通讯机构：[1]Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China [2]Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China
推荐引用方式(GB/T 7714)：
Zhang Huicun,Ta Na,Chen Pengmin,et al.Erchen Decoction and Linguizhugan Decoction Ameliorate Hepatic Insulin Resistance by Inhibiting IRS-1Ser307 Phosphorylation In Vivo and In Vitro[J].EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE.2017,2017:doi:10.1155/2017/1589871.
APA：
Zhang, Huicun,Ta, Na,Chen, Pengmin&Wang, Hongbing.(2017).Erchen Decoction and Linguizhugan Decoction Ameliorate Hepatic Insulin Resistance by Inhibiting IRS-1Ser307 Phosphorylation In Vivo and In Vitro.EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE,2017,
MLA：
Zhang, Huicun,et al."Erchen Decoction and Linguizhugan Decoction Ameliorate Hepatic Insulin Resistance by Inhibiting IRS-1Ser307 Phosphorylation In Vivo and In Vitro".EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE 2017.(2017)
