单位：[1]Department of Hematology, The Chinese PLA General Hospital, Beijing 100853, China[2]Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China
Occurrence of MLL (Mixed Lineage Leukemia) gene rearrangements indicates poor prognosis in acute myeloid leukemia (AML) patients. This is the first study to report the positive rate and distribution characteristics of MLL rearrangements in AML patients in north China. We used multiplex nested real time PCR (RT-PCR) to screen for incidence of 11 MLL rearrangements in 433 AML patients. Eleven MLL rearrangements included (MLL-PTD, MLL-AF9, MLL-ELL, MLL-AF10, MLL-AF17, MLL-AF6, MLL-ENL, MLL-AF1Q, MLL-CBP, MLL-AF1P, MLL-AFX1). There were 68 AML patients with MLL rearrangements, and the positive rate was 15.7%. MLL-PTD (4.84%) was detected in 21 patients, MLL-AF9 in 15, (3.46%), MLL-ELL in 10 (2.31%), MLL-AF10 in 8 (1.85%), MLL-AF1Q in 2 (0.46%), 3 cases each of MLL-AF17, MLL-AF6, MLL-ENL (0.69% each), a and single case each of MLL-CBP, MLL-AF1P, and MLL-AFX1 (0.23% each). The highest rate of MLL rearrangements was found in 24 patients with M5 subtype AML, occurring in 24 cases (35.3%). MLL rearrangements occurred in 21 patients with M2 subtype AML (30.9%), and in 10 patients with M4 subtype AML (14.7%). Screening fusion genes by multiplex nested RT-PCR is a convenient, fast, economical, and accurate method for diagnosis and predicting prognosis of AML.
基金：
China Postdoctoral FoundationChina Postdoctoral Science Foundation [2015M57275]; National Nature Science Foundations of ChinaNational Natural Science Foundation of China (NSFC) [81400135]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2016]版：
大类|4 区医学
小类|4 区工程：生物医学4 区卫生保健与服务
最新[2025]版：
大类|4 区医学
小类|4 区工程：生物医学4 区卫生保健与服务
JCR分区：
出版当年[2015]版：
Q4HEALTH CARE SCIENCES & SERVICESQ4ENGINEERING, BIOMEDICAL
最新[2023]版：
Q3HEALTH CARE SCIENCES & SERVICESQ4ENGINEERING, BIOMEDICAL
第一作者单位：[1]Department of Hematology, The Chinese PLA General Hospital, Beijing 100853, China[*1]Department of Hematology, The Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China
通讯作者：
通讯机构：[1]Department of Hematology, The Chinese PLA General Hospital, Beijing 100853, China[*1]Department of Hematology, The Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China
推荐引用方式(GB/T 7714)：
Yang Hua,Cao Tingting,Gao Li,et al.The incidence and distribution characteristics of MLL rearrangements in Chinese acute myeloid leukemia patients by multiplex nested RT-PCR[J].TECHNOLOGY and HEALTH CARE.2017,25:S259-S265.doi:10.3233/THC-171329.
APA：
Yang, Hua,Cao, Tingting,Gao, Li,Wang, Lili,Zhu, Chengying...&Yu, Li.(2017).The incidence and distribution characteristics of MLL rearrangements in Chinese acute myeloid leukemia patients by multiplex nested RT-PCR.TECHNOLOGY and HEALTH CARE,25,
MLA：
Yang, Hua,et al."The incidence and distribution characteristics of MLL rearrangements in Chinese acute myeloid leukemia patients by multiplex nested RT-PCR".TECHNOLOGY and HEALTH CARE 25.(2017):S259-S265
