单位：[1]State Key Laboratory of Oral Diseases,West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China [2]China-Japan Friendship Hospital, Department of Stomatology, Beijing 100029, China [3]Department of Oral Implantology,West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
Collagen is an essential component of the extracellular matrix (ECM) and is a suitable material for nerve repair during tissue remodeling for fracture repair. Epigallocatechin-3-gallate (EGCG), an extract of green tea, shows various biological activities that are beneficial to nerve repair. Here, we developed modified collagen containing different concentrations of EGCG (0.0064%, 0.064%, and 0.64%, resp.) to induce Schwann cell proliferation and differentiation. Cell Counting Kit-8 test, live/dead assay, and SEM showed that collagen cross-linked by EGCG induced Schwann cell proliferation. Real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blotting revealed that EGCG-modified collagen induced Schwann cell differentiation and downregulated reactive oxygen species (ROS) levels by downregulating the MAPK P38 signaling pathway. Our results indicate that collagen cross-linked with an appropriate concentration of EGCG induces the proliferation and differentiation of Schwann cells. The EGCG-modified collagen membrane may be applicable for nerve repair and guided tissue regeneration applications.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81671023]; National Key Research Development Program of China [2016YFA0201703/2016YFA0201700]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2016]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2015]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]State Key Laboratory of Oral Diseases,West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
通讯作者：
通讯机构：[1]State Key Laboratory of Oral Diseases,West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China [3]Department of Oral Implantology,West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
推荐引用方式(GB/T 7714)：
Chu Chenyu,Deng Jia,Cao Cong,et al.Evaluation of Epigallocatechin-3-gallate Modified Collagen Membrane and Concerns on Schwann Cells[J].BIOMED RESEARCH INTERNATIONAL.2017,2017:doi:10.1155/2017/9641801.
APA：
Chu, Chenyu,Deng, Jia,Cao, Cong,Man, Yi&Qu, Yili.(2017).Evaluation of Epigallocatechin-3-gallate Modified Collagen Membrane and Concerns on Schwann Cells.BIOMED RESEARCH INTERNATIONAL,2017,
MLA：
Chu, Chenyu,et al."Evaluation of Epigallocatechin-3-gallate Modified Collagen Membrane and Concerns on Schwann Cells".BIOMED RESEARCH INTERNATIONAL 2017.(2017)
