单位：[1]National Clinical Research Centre for Respiratory Diseases, Beijing Hospital, Beijing 100730, China.[2]Department of Surgical Intensive Care Medicine, Beijing An Zhen Hospital, Capital Medical University, No. 2 Anzhen Rd., Chao‑Yang District, Beijing 100029, China.首都医科大学附属安贞医院[3]Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, 2 Yinghua Dongjie, Beijing 100029, China.[4]Department of Pulmonary and Critical Care Medicine, Beijing Hospital, Beijing 100730, China.[5]Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing, China.
Background: Blood purification is an emerging approach to dampening the cytokine storm. This study aims to assess the efficacy of HA330 resin-directed hemoadsorption (HA) on endotoxin-induced porcine acute respiratory distress syndrome (ARDS) model. Methods: Twenty-four Chinese domestic pigs were allocated into saline group receiving intravenous infusion of saline (N = 6) and endotoxin group receiving intravenous infusion of LPS (N = 18). When ALI model was initially diagnosed, six pigs in the LPS and saline group were killed for BALF and histopathological analysis. The remaining 12 pigs in LPS group received 3-h HA (N = 6) or HA-sham (N = 6) treatment, respectively. Following another 5-h observation, animals were killed. Variables on hemodynamics, blood gases and lung mechanics were recorded at a series of time points. Differentially expressed cytokines and proteins were determined by ELISA and proteomics. Results: HA treatment significantly improved injured oxygenation induced by LPS. HA also partially improved the barrier permeability and reduced lung edema and inflammation/injury induced by LPS infusion. Proteomic analysis showed the differentially expressed proteins between HA-and HA-sham-treated groups mostly belonged to the categories of acute inflammation/immune response, and proteolysis. Conclusions: Hemoadsorption improved ARDS possibly by blunting the cytokine storm and by restoring homeostasis of the disordered proteome milieu in the exudative phase.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81490534, 81490530, 81430001]
第一作者单位：[1]National Clinical Research Centre for Respiratory Diseases, Beijing Hospital, Beijing 100730, China.[2]Department of Surgical Intensive Care Medicine, Beijing An Zhen Hospital, Capital Medical University, No. 2 Anzhen Rd., Chao‑Yang District, Beijing 100029, China.
共同第一作者：
通讯作者：
通讯机构：[1]National Clinical Research Centre for Respiratory Diseases, Beijing Hospital, Beijing 100730, China.[3]Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, 2 Yinghua Dongjie, Beijing 100029, China.
推荐引用方式(GB/T 7714)：
Xu Xuefeng,Jia Chune,Luo Sa,et al.Effect of HA330 resin-directed hemoadsorption on a porcine acute respiratory distress syndrome model[J].ANNALS of INTENSIVE CARE.2017,7:doi:10.1186/s13613-017-0287-0.
APA：
Xu, Xuefeng,Jia, Chune,Luo, Sa,Li, Yanming,Xiao, Fei...&Wang, Chen.(2017).Effect of HA330 resin-directed hemoadsorption on a porcine acute respiratory distress syndrome model.ANNALS of INTENSIVE CARE,7,
MLA：
Xu, Xuefeng,et al."Effect of HA330 resin-directed hemoadsorption on a porcine acute respiratory distress syndrome model".ANNALS of INTENSIVE CARE 7.(2017)
：
医学