单位：[1]Departmentof Hematology,China-Japan Friendship Hospital,Beijing,China[2]Departmentof Hematology andOncology,The First Aff|liated Hospital of Chinese PLAGeneral Hospital,Beijing,China[3]Department of Oncology,Chinese PLAGeneral Hospital,Beijing,China
The CpG island of the promoter region of the checkpoint with fork-head associated and ring finger gene (CHFR), a mitotic checkpoint gene with tumor-suppressor functions, is hypermethylated in various human cancers. The objective of this study was to evaluate the frequency of aberrant CHFR promoter methylation in acute myeloid leukemia (AML) patients in an attempt to improve prognostication. CHFR promoter methylation levels were analyzed in 358 newly diagnosed AML cases and 30 healthy donors by the use of quantitative methylation-specific polymerase chain reaction. In addition, we analyzed possible association between CHFR hypermethylation and hematological characteristics, chromosome abnormalities, genetic mutations, and survival. Hypermethylation of the CHFR promoter was observed in 24% (85 of 358) AML patients, but not in healthy individuals. CHFR hypermethylation correlated significantly with SRSF2 and DNMT3A mutations. Patients with hypermethylation exhibited lower overall survival and shorter relapse-free survival than nonmethylated cases. In multivariate analysis, CHFR hypermethylation was an independent factor predicting poor overall survival but not relapse-free survival. In conclusion, hypermethylation of the CHFR promoter, frequent in AML, is associated with adverse outcome, and can thus be used for risk stratification. (C) 2015 Wiley Periodicals, Inc.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81300425, 81300450]; Major State Basic Research Development Program of ChinaNational Basic Research Program of China [2005CB522400]; Key Research Program for Clinical High-Tech Research of People's Liberation Army [2010gxjs091]
通讯机构：[1]Departmentof Hematology,China-Japan Friendship Hospital,Beijing,China[2]Departmentof Hematology andOncology,The First Aff|liated Hospital of Chinese PLAGeneral Hospital,Beijing,China[*1]Department of Hematology, China-Japan Friendship Hospital, Yinghua East Road, Beijing 100029, China.[*2]Department of Hematology and Oncology, The First Affiliated Hospital of Chinese PLA General Hospital, Fucheng Road, Beijing 100048, China
推荐引用方式(GB/T 7714)：
Gao Li,Liu Fang,Zhang Hui,et al.CHFR Hypermethylation, a Frequent Event in Acute Myeloid Leukemia, Is Independently Associated with an Adverse Outcome[J].GENES CHROMOSOMES & CANCER.2016,55(2):158-168.doi:10.1002/gcc.22322.
APA：
Gao, Li,Liu, Fang,Zhang, Hui,Sun, Junzhong&Ma, Yigai.(2016).CHFR Hypermethylation, a Frequent Event in Acute Myeloid Leukemia, Is Independently Associated with an Adverse Outcome.GENES CHROMOSOMES & CANCER,55,(2)
MLA：
Gao, Li,et al."CHFR Hypermethylation, a Frequent Event in Acute Myeloid Leukemia, Is Independently Associated with an Adverse Outcome".GENES CHROMOSOMES & CANCER 55..2(2016):158-168
