单位：[1]Department of Thoracic Surgery, China–Japan Friendship Hospital, Beijing 100029, China[2]Department of Pathology, Harbor-UCLA Medical Center, 1000 West Carson Street, Torrance, CA 90502, USA
Aberrant promoter hypermethylations of tumor suppressor genes are promising markers for lung cancer diagnosis and prognosis. The purpose of this study was to determine methylation status at APC and RAR-beta promoters in primary NSCLC, and whether they have any relationship with survival. APC and RAR-beta promoter methylation status were determined in 41 NSCLC patients using methylation specific PCR. APC promoter methylation was detectable in 9 (22.0%) tumor samples and 6 (14.6%) corresponding non-tumor samples (P = 0.391). RAR-beta promoter methylation was detectable in 13 (31.7%) tumor samples and 4 (9.8%) corresponding non-tumor samples (P = 0.049) in the NSCLC patients. APC promoter methylation was found to be associated with T stage (P = 0.046) and nodal status (P = 0.019) in non-tumor samples, and with smoking (P = 0.004) in tumor samples. RAR-beta promoter methylation was found associated with age (P = 0.031) in non-tumor samples and with primary tumor site in tumor samples. Patients with APC promoter methylation in tumor samples showed significantly longer survival than patients without it (Log-rank P = 0.014). In a multivariate analysis of prognostic factors, APC methylation in tumor samples was an independent prognostic factor (P = 0.012), as were N1 positive lymph node number (P = 0.025) and N2 positive lymph node number (P = 0.06). Our study shows that RAR-beta methylation detected in lung tissue may be used as a predictive marker for NSCLC diagnosis and that APC methylation in tumor sample may be a useful marker for superior survival in NSCLC patients. (C) 2015 Published by Elsevier Inc.
基金：
National Key Clinical Specialty Construction Program [[2011]873]
第一作者单位：[1]Department of Thoracic Surgery, China–Japan Friendship Hospital, Beijing 100029, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, China–Japan Friendship Hospital, Beijing 100029, China[*1]Department of Thoracic Surgery, China–Japan Friendship Hospital, No.2 Yinghua East Road, Chaoyang District, Beijing 100029, China
推荐引用方式(GB/T 7714)：
Feng Hongxiang,Zhang Zhenrong,Qing Xin,et al.Promoter methylation of APC and RAR-beta genes as prognostic markers in non-small cell lung cancer (NSCLC)[J].EXPERIMENTAL and MOLECULAR PATHOLOGY.2016,100(1):109-113.doi:10.1016/j.yexmp.2015.12.005.
APA：
Feng, Hongxiang,Zhang, Zhenrong,Qing, Xin,Wang, Xiaowei,Liang, Chaoyang&Liu, Deruo.(2016).Promoter methylation of APC and RAR-beta genes as prognostic markers in non-small cell lung cancer (NSCLC).EXPERIMENTAL and MOLECULAR PATHOLOGY,100,(1)
MLA：
Feng, Hongxiang,et al."Promoter methylation of APC and RAR-beta genes as prognostic markers in non-small cell lung cancer (NSCLC)".EXPERIMENTAL and MOLECULAR PATHOLOGY 100..1(2016):109-113
