单位：[1]Department of Otorhinolaryngology—Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China,首都医科大学附属同仁医院[2]Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, 68178, United States of America,[3]Department of Otorhinolaryngology—Head and Neck Surgery, University of Nebraska Medical Center, Omaha, NE, 68198, United States of America,[4]Department of Otorhinolaryngology—Head and Neck Surgery, Chinese PLA General Hospital, Beijing, 100853, China,[5]Department of Pathophysiology, Second Military Medical University, Shanghai, 200433, China,[6]Department of Otorhinolaryngology—Head and Neck Surgery, Beijing Friendship Hospital, Beijing, China首都医科大学附属北京友谊医院
Regulation of gene expression is essential to determining the functional complexity and morphological diversity seen among different cells. Transcriptional regulation is a crucial step in gene expression regulation because the genetic information is directly read from DNA by sequence-specific transcription factors (TFs). Although several mouse TF data-bases created from genome sequences and transcriptomes are available, a cell type-specific TF database from any normal cell populations is still lacking. We identify cell type-specific TF genes expressed in cochlear inner hair cells (IHCs) and outer hair cells (OHCs) using hair cell-specific transcriptomes from adult mice. IHCs and OHCs are the two types of sensory receptor cells in the mammalian cochlea. We show that 1,563 and 1,616 TF genes are respectively expressed in IHCs and OHCs among 2,230 putative mouse TF genes. While 1,536 are commonly expressed in both populations, 73 genes are differentially expressed (with at least a twofold difference) in IHCs and 13 are differentially expressed in OHCs. Our datasets represent the first cell type-specific TF databases for two populations of sensory receptor cells and are key informational resources for understanding the molecular mechanism underlying the biological properties and phenotypical differences of these cells.
基金：
NIH from the NIDCD [R01 DC004696]; Major State Basic Research Development Program of China (973 Program)National Basic Research Program of China [2011CB504503]; NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Deafness & Other Communication Disorders (NIDCD) [R01DC004696] Funding Source: NIH RePORTER
第一作者单位：[1]Department of Otorhinolaryngology—Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China,[2]Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, 68178, United States of America,
通讯作者：
推荐引用方式(GB/T 7714)：
Yi Li,Huizhan Liu,Cody L. Barta,et al.Transcription Factors Expressed in Mouse Cochlear Inner and Outer Hair Cells[J].PLOS ONE.2016,11(3):doi:10.1371/journal.pone.0151291.
APA：
Yi Li,Huizhan Liu,Cody L. Barta,Paul D. Judge,Lidong Zhao...&David Z. Z. He.(2016).Transcription Factors Expressed in Mouse Cochlear Inner and Outer Hair Cells.PLOS ONE,11,(3)
MLA：
Yi Li,et al."Transcription Factors Expressed in Mouse Cochlear Inner and Outer Hair Cells".PLOS ONE 11..3(2016)
