Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-beta 1 expression
单位：[1]Department of Nephrology, Guang'Anmen Hospital of China Academy of Traditional Chinese Medicine Sciences,Beijing 100053[2]Beijing University of Chinese Medicine, Chaoyang, Liaoning 100029[3]Department of Pharmacology,Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, P.R. China
The aim of the present study was to investigate the effect of Yi Qi Qing Re Gao-containing serum (YQ-S) on rat mesangial cell (MC) proliferation and to investigate the underlying mechanism. MCs were divided into the control, lipopolysaccharide (LPS)-stimulated, YQ-S and fosinopril-containing serum (For-S) groups, and cultured for 48 h. An MTT assay was used to evaluate the proliferation of MCs. In addition, reverse transcription-quantitative polymerase chain reaction and western blot analysis were conducted to detect the expression levels of Wnt4, beta-catenin and transforming growth factor (TGF)-beta 1 in MCs. The results indicated that YQ-S inhibited LPS-induced MC proliferation. The Wnt4 and TGF-beta 1 mRNA expression levels were reduced in the YQ-S group (P<0.01 or P<0.05). Furthermore, the Wnt4, beta-catenin and TGF-beta 1 protein expression levels were suppressed in the YQ-S group (P<0.01 or P<0.05). Therefore, YQ-S appears to inhibit MC proliferation, and its mechanism may involve the inhibition of the Wnt signaling pathway and downregulation of TGF-beta 1 expression.
基金：
National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81102588, 81473614]
第一作者单位：[1]Department of Nephrology, Guang'Anmen Hospital of China Academy of Traditional Chinese Medicine Sciences,Beijing 100053
共同第一作者：
通讯作者：
通讯机构：[3]Department of Pharmacology,Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, P.R. China[*1]Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, 2 Yinghua Dongjie, Beijing 100029, P.R. China
推荐引用方式(GB/T 7714)：
Yang Liping,Sun Xueyan,Zhan Yongli,et al.Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-beta 1 expression[J].EXPERIMENTAL and THERAPEUTIC MEDICINE.2016,11(4):1410-1416.doi:10.3892/etm.2016.3027.
APA：
Yang, Liping,Sun, Xueyan,Zhan, Yongli,Liu, Huijie,Wen, Yumin...&Li, Ping.(2016).Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-beta 1 expression.EXPERIMENTAL and THERAPEUTIC MEDICINE,11,(4)
MLA：
Yang, Liping,et al."Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-beta 1 expression".EXPERIMENTAL and THERAPEUTIC MEDICINE 11..4(2016):1410-1416
