单位：[1]Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China临床科室口腔科口腔科首都医科大学附属北京友谊医院[2]Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China[3]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100871, China[4]Department of Thoracic Surgery, Sino-Japan Friendship Hospital, Beijing 100029, China[5]Biomics Biotechnologies Co., Ltd., Nantong 226016, China[6]Peking University, Beijing 100191, China
MicroRNAs are short endogenous non-coding RNAs that regulate gene expression in various physiological and pathological conditions. To characterize autoantigen-targeting microRNAs in Sjogren's syndrome (SS), a systematic study was carried out, in which a candidate microRNA set was first identified by bioinformatics analysis and literature search. Then, their gene silencing activities were evaluated with fusion reporter gene and endogenous targets, leading to the identification of three microRNAs: TRIM21-targeting miR-1207-5p, TRIM21-targeting miR-4695-3p, and La autoantigen-targeting miR-299-5p. Compared to healthy controls, downregulation of miR-1207-5p and miR-4695-3p expression was further revealed in the minor salivary glands of primary SS (pSS) patients. This, on the one hand, characterized two autoantigen-targeting microRNAs in Sjogren's syndrome and, on the other hand, suggested that downregulation of miR-1207-5p and miR-4695-3p expression may lead to increased TRIM21 levels in the minor salivary glands, which contributes to the development of Sjogren's syndrome.
基金：
Beijing Municipal Administration of Hospitals Incubating Program [PX2016065]; Nantong Jianghai Talent Program; National High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2011AA020111]; National Science Technology Pillar Program [2008BAI59B03]; Special Research Fund for Public Welfare and the Health Industry [201202004]; National High-tech R&D Program of China [2012AA022501]
第一作者单位：[1]Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
共同第一作者：
通讯作者：
通讯机构：[3]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100871, China[5]Biomics Biotechnologies Co., Ltd., Nantong 226016, China[6]Peking University, Beijing 100191, China
推荐引用方式(GB/T 7714)：
Yang Ying,Peng Linyi,Ma Weizhi,et al.Autoantigen-targeting microRNAs in Sjogren's syndrome[J].CLINICAL RHEUMATOLOGY.2016,35(4):911-917.doi:10.1007/s10067-016-3203-3.
