单位：[1]School of Food Science and Engineering, Harbin Institute of Technology, Harbin, 150090 China[2]School of Food Science and Engineering, Harbin Institute of Technology, Harbin, 150090 China[3]Department of Computer Science, Georgia State University, Atlanta, GA 30303 USA[4]Orthodontic Department, China-Japan Friendship Hospital, Beijing, China[5]Department of Geriatrics, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150000, China
Chemotherapy is the main strategy in the treatment of cancer; however, the development of drug-resistance is the obstacle in long-term treatment of cervical cancer. Cisplatin is one of the most common drugs used in cancer therapy. Recently, accumulating evidence suggests that miRNAs are involved in various bioactivities in oncogenesis. It is not unexpected that miRNAs play a key role in acquiring of drug-resistance in the progression of tumor. In this study, we induced and maintained four levels of cisplatin-resistant HeLa cell lines (HeLa/CR1, HeLa/CR2, HeLa/CR3, and HeLa/CR4). According to the previous studies and existing evidence, we selected five miRNAs (miR-183, miR-182, miR30a, miR-15b, and miR-16) and their potential target mRNAs as our research targets. The real-time RT-PCR was adopted to detect the relative expression of miRNAs and their mRNAs. The results show that miR-182 and miR-15b were up-regulated in resistant cell lines, while miR-30a was significantly down-regulated. At the same time, their targets are related to drug resistance. Compared to their parent HeLa cell line, the expression of selected miRNAs in resistant cell lines altered. The alteration suggests that HeLa cell drug resistance is associated with distinct miRNAs, which indicates that miRNAs may be one of the therapy targets in the treatment of cervical cancer by sensitizing cell to chemotherapy. We suggested a possible network diagram based on the existing theory and the preliminary results of candidate miRNAs and their targets in HeLa cells during development of drug resistance.
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2015]版：
大类|3 区工程技术
小类|4 区生化研究方法4 区纳米科技
最新[2025]版：
大类|3 区生物学
小类|3 区生化研究方法4 区纳米科技
JCR分区：
出版当年[2014]版：
Q2NANOSCIENCE & NANOTECHNOLOGYQ3BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q2BIOCHEMICAL RESEARCH METHODSQ3NANOSCIENCE & NANOTECHNOLOGY
第一作者单位：[1]School of Food Science and Engineering, Harbin Institute of Technology, Harbin, 150090 China
通讯作者：
通讯机构：[1]School of Food Science and Engineering, Harbin Institute of Technology, Harbin, 150090 China[*1]Xi’an Jiaotong- Liverpool University, Suzhou, 215123 China
推荐引用方式(GB/T 7714)：
Yang Yubo,Dai Cuihong,Cai Zhipeng,et al.The Pathway Analysis of Micrornas Regulated Drug-Resistant Responses in HeLa Cells[J].IEEE TRANSACTIONS on NANOBIOSCIENCE.2016,15(2):115-120.doi:10.1109/TNB.2016.2539365.
APA：
Yang, Yubo,Dai, Cuihong,Cai, Zhipeng,Hou, Aiju,Cheng, Dayou...&Xu, Dechang.(2016).The Pathway Analysis of Micrornas Regulated Drug-Resistant Responses in HeLa Cells.IEEE TRANSACTIONS on NANOBIOSCIENCE,15,(2)
MLA：
Yang, Yubo,et al."The Pathway Analysis of Micrornas Regulated Drug-Resistant Responses in HeLa Cells".IEEE TRANSACTIONS on NANOBIOSCIENCE 15..2(2016):115-120
