Validation and comparison of a rapid liquid chromatography tandem mass spectrometry method for serum 250HD with the efficiency of separating 3-epi 250HD(3)
单位：[1]Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China[2]Beijing Hospital National Center for Clinical Laboratories, Ministry of Health, Beijing 100730, China[3]Department of Clinical Laboratory, China–Japan Friendship Hospital, Beijing 100029, China
Objectives: To develop a rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method with ability to separate 3-epi 250HD(3) (EPI-LC-MS/MS) from 250HD(3), and evaluate the effects of 3-epi 250HD(3) on routine LC-MS/MS that cannot separate 3-epi 250HD(3) (NEPI-LC-MS/MS). Design and methods: Performance of the newly built EPI-LC-MS/MS was validated, and 982 samples were analyzed and compared by the two methods. Results: Both methods showed a linearity coefficient correlation exceeding 0.999 in the 6.25-500 nmol/L range for 250HD(2) and 250HD(3). Moreover, they showed a between run coefficient variation (CV) and total CV of < 5% for 250HD(2) and 250HD(3). The results of the accuracy test showed that the bias was below 6.19% in the absence of 3-epi 250HD(3). Comparison of the 250HD results obtained by the two methods for 982 patients (age 1-100 years) revealed excellent clinical agreement (Cohen's kappa = 0.875) and correlation (R2 = 0.973). Among the 982 patients, only 73 patients had 3-epi 250HD(3) (> 6.25 nmol/L); out of these 73 patients, the 3-epi 250HD(3) level in 58 patients was between 6.25 and 12.5 nmol/L. In patients with < 375 nmol/L 250HD (250HD(2) + 250HD(3)), only 8 had 3-epi 250HD(3) levels exceeding 12.5 nmol/L (range: 13.3 - 27.5 nmol/L). Among samples containing 3-epi 250HD(3), only three were separated into different 250HD-deficiency groups using the above methods. Conclusion: A rapid and precise EPI-LC-MS/MS method for measuring 250HD with efficient separation of 3-epi 250HD(3) was developed. Our results showed that 3-epi 250HD(3) had little effect on the routinely used NEPI-LC-MS/MS. (C) 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
基金：
China National Clinical Key Subject Program; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81201337, 81171665]
第一作者单位：[1]Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China[*1]Department of Clinical Laboratory，Peking Union Medical College Hospital, Chinese Academy of Medical Sciences. No. 1 Shuaifu yuan, Dongcheng District, Beijing 100730, PR China.
推荐引用方式(GB/T 7714)：
Yu Songlin,Zhou Weiyan,Zhang Ruiping,et al.Validation and comparison of a rapid liquid chromatography tandem mass spectrometry method for serum 250HD with the efficiency of separating 3-epi 250HD(3)[J].CLINICAL BIOCHEMISTRY.2016,49(13-14):1004-1008.doi:10.1016/j.clinbiochem.2016.05.011.
APA：
Yu, Songlin,Zhou, Weiyan,Zhang, Ruiping,Cheng, Xinqi,Tang, Huiling...&Qiu, Ling.(2016).Validation and comparison of a rapid liquid chromatography tandem mass spectrometry method for serum 250HD with the efficiency of separating 3-epi 250HD(3).CLINICAL BIOCHEMISTRY,49,(13-14)
MLA：
Yu, Songlin,et al."Validation and comparison of a rapid liquid chromatography tandem mass spectrometry method for serum 250HD with the efficiency of separating 3-epi 250HD(3)".CLINICAL BIOCHEMISTRY 49..13-14(2016):1004-1008
