Expression patterns of T-type Ca(v)3.2 channel and insulin-like growth factor-1 receptor in dorsal root ganglion neurons of mice after sciatic nerve axotomy
单位：[1]Department of Anesthesiology, China-Japan Friendship Hospital, Beijing, China[2]Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, China[3]State Key Laboratory of Neuroscience, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, CAS Center for Excellence in Brain Science & Intelligence Technology, Chinese Academy of Sciences, Shanghai, China
Substantial evidence indicates that T-type Ca(v)3.2 channel and insulin-like growth factor-1 (IGF-1) contribute to pain hypersensitivity within primary sensory nerves. A recent study suggested that activation of IGF-1 receptor (IGF-1R) could increase Ca(v)3.2 channel currents and further contribute to inflammatory pain sensitivity. However, the expression patterns of Ca(v)3.2 and IGF-1R and their colocalization in dorsal root ganglion (DRG) in chronic neuropathic pain condition remain unknown. In this study, we explored expression patterns of Ca(v)3.2, IGF-1R and their colocalization, and whether phenotypic switch occurs in a subpopulation of Ca(v)3.2(+) or IGF-1R(+) neurons in mouse DRGs after sciatic nerve axotomy with immunofluorescence, real-time reverse transcription-PCR, and western blot assays. We found that expressions of Ca(v)3.2 and IGF-1R, and their colocalization were not increased in DRGs of mice following axotomy. In addition, Ca(v)3.2(+) or IGF-1R(+) subpopulation neurons did not acquire significant switch in expression phenotype after sciatic nerve axotomy. Our findings argue for an upregulation of Ca(v)3.2 and IGF-1R expression in lumbar DRGs post-sciatic nerve axotomy and provided an insight for understanding the functions of peripheral afferent Ca(v)3.2 channel and IGF-1/IGF-1R signaling in chronic neuropathic pain.
基金：
research projects of the hospital of China-Japan Friendship Hospital [2015-1-QN-18]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371122, 81322015, 30870832]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB02010000]
第一作者单位：[1]Department of Anesthesiology, China-Japan Friendship Hospital, Beijing, China[2]Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, China
共同第一作者：
通讯作者：
通讯机构：[2]Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, China[*1]Xuzhou Medical University, D439, 209 Tongshan Road, Xuzhou, Jiangsu 221004, China
推荐引用方式(GB/T 7714)：
Lin Si-Fang,Yu Xiao-Lu,Liu Xiao-Ya,et al.Expression patterns of T-type Ca(v)3.2 channel and insulin-like growth factor-1 receptor in dorsal root ganglion neurons of mice after sciatic nerve axotomy[J].NEUROREPORT.2016,27(15):1174-1181.doi:10.1097/WNR.0000000000000676.
APA：
Lin, Si-Fang,Yu, Xiao-Lu,Liu, Xiao-Ya,Wang, Bing,Li, Cheng-Hui...&Liu, Xing-Jun.(2016).Expression patterns of T-type Ca(v)3.2 channel and insulin-like growth factor-1 receptor in dorsal root ganglion neurons of mice after sciatic nerve axotomy.NEUROREPORT,27,(15)
MLA：
Lin, Si-Fang,et al."Expression patterns of T-type Ca(v)3.2 channel and insulin-like growth factor-1 receptor in dorsal root ganglion neurons of mice after sciatic nerve axotomy".NEUROREPORT 27..15(2016):1174-1181
