单位：[1]Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China临床科室心血管中心心内科首都医科大学附属北京友谊医院[2]Department of Infection, Beijing Friendship Hospital, Capital Medical University, Beijing, China临床科室急危重症及感染医学中心感染内科首都医科大学附属北京友谊医院[3]Department of Cardiology, Hangzhou First People’s Hospital, Zhejiang, China[4]Department of Cardiology, Baotou Central Hospital, Inner Mongolia, China
IntroductionCardiac cell apoptosis plays a crucial role in the progression of diabetic cardiomyopathy. Recent studies have shown that fasudil, a Rho-kinase (ROCK) inhibitor, inhibits cardiac cell apoptosis; however, the underlying mechanism remains unclear. AimThis study aimed to investigate whether fasudil protects H9c2 cells from high glucose-induced apoptosis via activation of autophagy. MethodsRat cardiomyocyte H9c2 cells were treated with high glucose and used as a diabetic cardiomyopathy model. Cell survival rate, apoptosis, and subcellular morphology were examined using the MTT assay, flow cytometry, and electron microscopy, respectively. ROCK1 and ROCK2 mRNA levels were determined using quantitative real-time PCR. Bcl-2 and Bax, myosin phosphatase target subunit-1 (MYPT-1), phosphorylated (p)-MYPT1, LC3-II/LC3-I, Beclin-1, soluble and insoluble P62 protein levels were determined by Western blot analysis. ResultsFasudil reversed the high glucose-induced inhibition of cell proliferation and suppressed high glucose-induced early apoptosis. Fasudil also reversed the high glucose-suppressed Bcl-2 levels and decreased the high glucose-induced Bax levels. Further, Fasudil suppressed ROCK levels, expression, promoted autophagy via increasing the LC3-II/LC3-I ratio, Beclin-1 expression, and the number of autophagosomes in H9c2 cells treated with high glucose. These effects of fasudil were abrogated by 3-methyladenine (3-MA), an autophagy inhibitor. ConclusionFasudil inhibited high glucose-induced apoptosis in rat H9c2 cells through activating autophagy.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570220]
第一作者单位：[1]Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[1]Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China[*1]Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Xicheng, Beijing, China.
推荐引用方式(GB/T 7714)：
Gao Huikuan,Hou Fei,Dong Ruiqing,et al.Rho-Kinase inhibitor fasudil suppresses high glucose-induced H9c2 cell apoptosis through activation of autophagy[J].CARDIOVASCULAR THERAPEUTICS.2016,34(5):352-359.doi:10.1111/1755-5922.12206.
APA：
Gao, Huikuan,Hou, Fei,Dong, Ruiqing,Wang, Zefeng,Zhao, Can...&Wu, Yongquan.(2016).Rho-Kinase inhibitor fasudil suppresses high glucose-induced H9c2 cell apoptosis through activation of autophagy.CARDIOVASCULAR THERAPEUTICS,34,(5)
MLA：
Gao, Huikuan,et al."Rho-Kinase inhibitor fasudil suppresses high glucose-induced H9c2 cell apoptosis through activation of autophagy".CARDIOVASCULAR THERAPEUTICS 34..5(2016):352-359
